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通过图位克隆鉴定了控制开发花蕾的ob1基因,是位于D13染色体上的MIXTA-like MYB基因,解释了ob2位点的因果变异。运用全基因组转录组分析技术,发现ob突变体中部分MYB基因和细胞壁生物发生、毛状体分化、细胞分裂素信号转导和细胞分裂的相关基因受到抑制,花瓣生长可能因此受到抑制。
系统鉴定和分析了棉花SUN家族蛋白,通过病毒诱导的敲除和CRISPR/cas9介导的GbSUN5敲除,发现尽管花粉活力仍然正常,但是其败育率较高。本研究结果表明:SUN3和SUN5在生物学功能上存在差异,在生殖发育中发挥重要作用。
本研究利用收集的436份国内外变异广泛的陆地棉材料,采用全基因组重测序方法,构建了群体的SNP变异图谱,通过对包含136份早熟棉资源在内的356份陆地棉材料进行深度重测序,并结合公共数据库中的80份陆地棉材料共获得了10,118,884个高质量的SNPs。利用全基因组关联分析(GWAS)鉴定到控制陆地棉早熟性的307个显著遗传位点和重要调控基因。两个早熟遗传热点区域位于 D03和A05&...
Two experiments were conducted to assess gender (Experiment 1) and age (Experiment 2) effects on the expression of genes related to lipid metabolism in broiler chickens. The expression of fatty acid ...
Reverse transcription quantitative real-time PCR (RT-qPCR) is widely used to study the relative abundance of mRNA transcripts because of its sensitivity and reliable quantification. However, the reli...
Site-directed mutagenesis is becoming a very important technique in the study of photosynthetic reaction center structure-function relationships. However, there are certain disadvantages associated wi...
The goal of this work was to investigate the correlations between MyHC mRNA transcription and their corresponding protein expressions in porcine longissimus muscle (LM) during postnatal growth of pigs...
We hypothesized that supplementing finishing diets with palm oil would promote adipogenic gene expression and stearoyl-CoA desaturase (SCD) gene expression in subcutaneous (s.c.) and intramuscular (i....
Although the chemical, physical, and nutritional properties of bovine milk have been extensively studied, only a few studies have attempted to characterize milk-synthesizing genes using RNA-seq data. ...
The objective of this study was to investigate the correlation between cattle breeds and deposit of adipose tissues in different positions and the gene expressions of peroxisome proliferator-activated...
The retinoids (vitamin A and its derivatives) play a critical role in vision, growth, reproduction, cell differentiation and embryonic development. Using the IMpRH panel, porcine cellular retinol bind...
Our earlier studies showed that estrogen was involved in the regulation of fluid reabsorption in adult mouse efferent ductules (ED), through estrogen receptor (ER) ??and ER?? by modulating gene expres...
Using the somatic cell hybrid panel (SCHP) and radiation hybrid (IMpRH) panel, porcine SOCS2 gene was mapped at SSC5 (1/2) q21-q24 and closely linked with SW1383 marker (47 cR in distance), while SOCS...
Predatory snails in the marine gastropod genus Conus stun prey by injecting a complex mixture of peptide neurotoxins. These conotoxins are associated with trophic diversification and block a divers...
A key tool in evolutionary ecology is information about the temporal dynamics of species over time. Paleontology has long been the major source of this information, however, a very different source ...

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