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Rapid and visual detection of 2019 novel coronavirus (SARS-CoV-2) by a reverse transcription loop-mediated isothermal amplification assay
SARS-CoV-2 RT-LAMP COVID-19 Detection Visual
2020/6/12
To evaluate a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), and compare it with RT-PCR.
Detection of Klebsiella pneumonia in respiratory tract samples by the loop-mediated isothermal amplification method
环介导等温扩增技术 呼吸道感染 肺炎克雷伯菌 细菌培养法 DNA测序技术
2021/3/29
建立环介导等温扩增技术（loop-mediated isothermal amplification，LAMP）检测呼吸道感染患者痰液中肺炎克雷伯菌（Klebsiella pneumoniae，Kpn）的方法并进行初步应用。 方法：基于Kpn phoE基因设计4条特异性引物，对反应条件中的Mg2+和甜菜碱浓度进行优化，建立检测Kpn LAMP方法并进行特异性和灵敏度试验；收集308例呼吸道感染患...
