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A Rapid Method of Complement Fixation Test for Syphilis
Rapid Method Complement Fixation Test Syphilis
2009/12/3
A rapid 'method for carrying out the complement fixation test for the diagnosis of syphilis involving stirring is descr-ibed.
Flat bottomed tubes each containing a glass bead are. employed and st i'r...
Doppler examination in varicocele. A standard method of evaluation
Doppler examination varicocele evaluation
2009/2/23
Doppler sonography is considered a reliable method for detecting reflux due to venous valvular incompetence, which occurs in varicocele. It is, however, a matter of debate whether the characteristic...
Quantification of bovine sperm separation by a swim-up method. Relationship to sperm motility, integrity of acrosomes, sperm migration in polyacrylamide gel and fertility
Quantification bovine sperm separation swim-up method
2009/2/23
The number of bovine spermatozoa separated in a swim-up procedure was quantified using an electronic cell counter. In an initial test of the swim-up procedure, non-frozen sperm samples with differen...
A Method for Improvement of Sperm Quality of an Ejaculated Human Specimen Using Bovine Cervical Mucus as a Biological Filter
teratozoospermia bovine cervical mucus penetration test
2009/2/19
A method is described for improving the quality of semen from subfertile men. Semen samples were filtered through bovine cervical mucus (collected at estrus) into spermatozoa-free seminal plasma (fr...
A Method for the Determination of Chlorinated Pesticides in Human Semen
chlorinated pesticide human semen gas chromatography
2009/2/19
A method is described for the quantitative and qualitative determination of BHC and DDT isomers and HCB and Methoxychlor (DMDT) in human semen. Pesticide residues were extracted with petroleum eth...
A Rapid and Simple Method for Isolating Principal Cells from the Rat Caput Epididymidis
epididymis principal cell rapid isolation
2009/2/19
A technique is described for the rapid isolation of principal cells from enzymatically dispersed rat caput epididymidis by differential sedimentation into calf serum. Sedimentations were prepared in...
A Continuous Flow Method for Organ Culture of Rabbit Epididymis：Morphology, Amino Acid Utilization, Glucose Uptake, RNA, and Protein Synthesis
epididymis organ culture glucose uptake amino acid utilization
2009/2/18
A modified perfusion system was developed to maintain an unconvoluted tubule from the corpus epididymidis of the rabbit in organ culture. The epididymal explant is submerged in a continuous flow of ...
Analysis of the effect of nitric oxide synthase inhibition on mouse sperm employing a modified staining method for assessment of the acrosome reaction
Nitric oxide synthase acrosome reaction
2009/2/17
A commercially available staining kit (Spermac) was combined with swelling in a hypoosmotic medium (HOS) for simultaneous assessment of viability and acrosome reaction in mouse spermatozoa. We com...
Effects of substrate and separation method on acrosin amidase measurements
Enzyme activity DL-BAPNA L-BAPNA swim-up
2009/2/16
The purpose of this study was to increase the accuracy and reproducibility of the acrosin amidase assay and to assess the effects of different methods of sperm isolation on total sperm acrosin act...
Simple, rapid staining method for immediate intraoperative examination of testicular biopsies
Azoospermia testicular biopsy testicular touch imprint
2009/2/16
Before patients with obstructive azoospermia undergo surgical reconstruction, testicular biopsy is required to establish that spermatogenesis is normal. To perform the corrective surgical procedur...
Influence of semen collection method on ejaculate characteristics in the common marmoset, Callithrix jacchus
Spermatozoa electroejaculation vaginal washing artificial insemination
2009/2/16
A source of normal spermatozoa (sperm) is required for many andrological and reproductive studies. Ideally the method used for semen collection should be repeatable and reliable but should not influ...
Acrosin activity of human spermatozoa by means of a simple gelatinolytic technique：a method useful for IVF
Human spermatozoa acrosin activity male infertility
2009/2/13
Acrosin activity was determined using a gelatinolysis technique in 100-microliter semen aliquots of 114 patients (normozoospermia, n = 90; asthenozoospermia, n = 12; oligozoospermia, n = 10; polyz...
A new method to generate canine seminal emission and its application to men：direct electrical stimulation of the vas deferens
generate canine seminal emission direct electrical stimulation vas deferens
2009/2/12
Seminal emission from the ejaculatory duct (SEED) by direct electrical stimulation of the vas deferens was investigated in the dog, and the technique was applied to men. The stimulus parameters used w...
A minor modification of the postcoital test (PCT) is made, which allows for a volumetric inspection of the cervical mucus with a standard clinical microscope. Homogeneously sized (40.3-microns) pl...
The fix vital stain method. Simultaneous determination of viability and acrosomal status of bovine spermatozoa
Acrosomal evaluation vital staining Hoechst bisbenzimide
2009/2/7
A rapid, accurate and precise method for simultaneous determination of acrosomal status and viability of bull spermatozoa is introduced and evaluated. The method involves fixation of semen with glut...
