搜索结果: 1-7 共查到“医学微生物学 PCR”相关记录7条 . 查询时间(0.187 秒)
lipL32-PCR应用于钩端螺旋体检测
外膜脂蛋白基因 钩端螺旋体 聚合酶链反应
2014/6/23
探讨lipL32-PCR检测方法 对钩端螺旋体(钩体)检测的可行性。方法 根据外膜脂蛋白基因(lipL32)设计引物,与卫生行业标准推荐的引物G1/G2对比,进行特异性与灵敏度研究,并应用于常山县蛙、鼠肾脏标本钩体PCR检测;对浙江省2008年分离菌株进行钩体PCR鉴定。结果lipL32-PCR具有较高的灵敏度和特异性,该引物可特异性扩增致病性钩体DNA,对本实验所用的其他细菌不扩增。2008年分...
Detection of Brucella by Peripheral Blood PCR and Comparison with Culture and Serological Methods in Suspected Cases
Brucella Culture and serological methods PCR
2009/12/17
Background: Brucellosis is a zoonotic disease and its symptoms and appearances are not exclusive in human and its traditional diagnosis is based on blood culture and serological methods. For more...
Single Strand Conformation Polymorphism analysis of PCR-amplified rDNA to differentiate medically important Aspergillus Species
SSCP Aspergillus Identification PCR
2009/12/9
Background: Aspergillus species are associated with allergic bronchopulmonary disease, mycotic keratitis, otomycosis, nasal sinusitis and invasive infection. In this study, we developed a PCR-Sin...
Comparison of Integrated Cell Culture -RT-PCR & Cell Culture Methods for Detection of Enteroviruses
Enteroviruses Sewage ICC-RT-PCR Iran
2009/11/26
Background: Generally, sewage exposed water could be potentially contaminated with enteroviruses. For this reason, enterovirus isolation from sewage specimens is one of the most sensitive indicat...
目的利用基于O抗原翻转酶wzx基因的PCR方法，鉴别与大肠埃希菌O157血清凝集的弗氏枸橼酸杆菌和O157大肠埃希菌。方法用微生物鉴定仪对细菌进行生化鉴定，与O157血清凝集，同时扩增wzx基因。结果25株与大肠埃希菌O157血清凝集的弗氏枸橼酸杆菌及1株不与大肠埃希菌O157血清凝集的弗氏枸橼酸杆菌wzx基因扩增为阳性，8株与O157血清不凝集的弗氏枸橼酸杆菌wzx基因扩增为阴性，20株O157...
目的：比较烟曲霉毒力相关基因（pksP、fos-1、rhbA、paba、lysf、cpcA）在体内和体外表达上的差异，探讨毒力因子在感染宿主时的表达模式以及这种表达差异在烟曲霉致病方面的重要意义。材料与方法：1 菌株：本实验所用菌株为BMU1200号烟曲霉，为临床分离株。2 体外培养菌丝RNA的提取：调节孢子浓度为106/ml，接种于AMM液体培养基，37 ℃ 、220rpm培养24小时，收集菌丝...
