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Lead induced alterations in rabbit spermatozoa motility and morphology in vitro
reproductive toxicity rabbit Pb temperature sperm motility morphological abnormality
2016/11/11
The aim of this in vitro study was to determine the effect of lead chloride (PbCl2) on rabbit spermatozoa motility and morphology. Lead concentrations in the medium ranged between 0.45 and 11.17 μg/ml...
Cryopreservation of fluorescence activated cell sorted boar spermatozoa based on extracellular ubiquitination
pig antibody staining quality marker porcine sperm FACS ubiquitin cryopreservation
2016/8/1
The present study is focused on the methodology of fluorescence activated cell sorting (FACS) of spermatozoa stained by the antibody against extracellular surface marker ubiquitin (eUb) and subsequent...
Kinetics of hydrogen peroxide generated from live and dead ram spermatozoa and the effects of catalase and oxidase substrates addition
reactive oxygen species ram antioxidant
2016/1/29
The generation of hydrogen peroxide (H2O2) by ram spermatozoa (spz) was measured using a flurometric assay with 10-acetyl-3,7-dihydroxyphenoxazine agent as a probe for H2O2 detection. The kinetics of ...
Relationship between acrosome integrity changes and in vitro fertilising ability of bovine spermatozoa
bull spermatozoa acrosome embryos IVF
2015/10/9
This study was designed to investigate the characteristics of acrosomal changes during capacitation of bovine spermatozoa in relationship to in vitro fertility of individual bulls. Motile spermatozoa ...
Effect of long-term storage on induced photon emission of boar spermatozoa
boar semen oxidative stress luminescence photon emission sperm motility
2015/6/2
Ultraweak photon emission measurements were found to be a useful tool for investigating the influence of stress factors on cells. In the present study, induced photon emission of boar spermatozoa was ...
DNA integrity in fresh, chilled and frozen-thawed canine spermatozoa
dog DNA fragmentation index sperm chromatin structure assay (SCSA) cryopreservation chilled semen
2015/4/16
Sperm chromatin status in fresh dog semen and the effect of long-term storage of chilled and frozen dog semen on sperm chromatin integrity was assessed by the sperm chromatin structure assay (SCSA). I...
Effect of acrosome reaction progress in frozen-thawed boar spermatozoa on the efficiency of in vitro oocyte fertilization
pig cryopreserved sperm assessment
2015/4/7
A good functional status of cryopreserved boar spermatozoa is very important for successful fertilization of porcine oocytes and in vitro embryo production. The purpose of the study was to evaluate th...
Determination of Acrosomal Proteolytic Activity in Spermatozoa Collected from Quail Treated with Diethylstilbestrol,Using Gelatin-Substrate Slide Technique
acrosomal proteolytic activity (APA) diethylstilbestrol gelatin-substrate slide quail sperm
2009/7/30
The present experiment was designed to ascertain whether gelatin-substrate slide technique devised for detection of the proteolytic activity of acrosomal contents can be used for quail sperm, and to d...
