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华中农业大学在植物Rubisco遗传改良方面取得新进展
华中农业大学 植物 Rubisco 遗传改良
2023/2/24
近日,华中农业大学生命科学技术学院、作物遗传改良全国重点实验林拥军教授团队联合英国利物浦大学刘鲁宁教授科研团队在利用细菌快速Rubisco优化高等植物光合作用领域取得新进展。相关研究结果以“Producing fast and active Rubisco in tobacco to enhance photosynthesis”为题在线发表在国际期刊The Plant Cell。
Endeavours of RuBisCO small subunit promoter as a tool of green tissue specific expression
constitutive expression rbcS promoter spatio-temporal expression
2014/2/25
Transcriptomics has the potential to rapidly increase our knowledge of spatial and temporal gene expression and contributes to the characterization of new promoters for research and development. The s...
Gene Expression and Accumulation of Rubisco in Bundle Sheath and Mesophyll Cells during Leaf Development and Senescence in Rice,a C3 Plant
Bundle sheath C3 photosynthesis Chlorophyll fluorescence Immuno-localization In situ hybridization Leaf development Leaf senescence Mesophyll
2009/7/23
Gene expression of ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) large subunit (rbcL) and small subunit (rbcS) in bundle sheath and mesophyll cells of rice, a C3 plant, was examined during...
The QTL Analysis of RuBisCO in Flag Leaves and Non-Structural Carbohydrates in Leaf Sheaths of Rice Using Chromosome Segment Substitution Lines and Backcross Progeny F2 Populations
Backcross progeny Chromosome segment substitution lines (CSSLs) Leaf sheath Non-structural carbohydrate (NSC) QTL Rice (Oryza sativa L.) RuBisCO
2009/7/21
In rice (Oryza sativa L.), the maintenance of high photosynthetic rate of flag leaves and the carbon remobilization from leaf sheaths after heading is a critical physiological component affecting the ...
由核基因编码的叶绿体前体蛋白能否正确定向和转运到叶绿体,对叶绿体的功能十分重要。采用酵母双杂交方法,筛选豌豆的cDNA表达文库,分析检测与Rubisco小亚基前体蛋白(preSSU)相互作用并参与其调控的蛋白。结果表明,共有6种不同类型的蛋白与preSSU互作,分别为组氨酸蛋白激酶基因、转运蛋白复合体、转录和翻译相关蛋白及复合体、信号传导相关基因和光合合成相关基因。通过与相关蛋白的相互作用,pre...
以水稻(Oryza sativa L. subsp. japonica)品种“中花11”构建的T-DNA插入突变体库为材料,用甲基紫精诱导叶绿体产生活性氧,通过SDS-PAGE电泳分析Rubisco含量相对于野生型的变化,筛选耐氧化胁迫的株系。结果表明,与对照相比,突变体材料各个株系叶片中的Rubisco小亚基的相对含量有较大差异,说明这种筛选Rubisco突变体的方法是可行的,且从中找到了两株R...
Rubisco活化酶基因反义表达载体的构建与水稻的遗传转化
水稻 Rubisco活化酶 反义 载体构建 遗传转化
2008/1/6
摘要利用pGEM-T easy 、pBluescript KS+获得了RCA基因两端可以引入与双元表达载体pCAMBIA1301多克隆位点相匹配的单酶切位点,将该片段连接到pCAMBIA1301载体,构建了RCA基因的反义植物表达载体pCAMR02。通过电激法将该载体导入根癌农杆菌EHA105菌株中,以根癌农杆菌介导的方法,将反义载体pCAMR02转化粳稻品种中花11,获得了抗潮霉素的再生植株,经...
小麦Rubisco活化酶的纯化及其活性特性
小麦 Rubisco活化酶 ATP
2010/3/10
试验以小麦为材料,纯化了Rubisco活化酶,经SDS 聚丙烯凝胶电泳鉴定呈 2条蛋白质谱带,分子量为410 0 0和 45 0 0 0 ,利用14 C标记法测定并探讨了Rubisco活化酶的活性,并发现Rubisco活化酶在纯化和储藏过程中都需要ATP以稳定其活性。