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Protocol: low cost fast and efficient generation of molecular tools for small RNA analysis
Vector Target mimic miRNA, Cloning Plant Downregulation
2024/1/19
Small RNAs are sequence-dependent negative regulators of gene expression involved in many relevant plant processes such as development, genome stability, or stress response. Functional characterizatio...
Combined fluorescent seed selection and multiplex CRISPR/Cas9 assembly for fast generation of multiple Arabidopsis mutants
Plant gene editing Fluorescent seed selection Co-transformation Cas9 zCas9i pEC1.2 PcUBi4-2
2023/11/30
Multiplex CRISPR-Cas9-based genome editing is an efficient method for targeted disruption of gene function in plants. Use of CRISPR-Cas9 has increased rapidly in recent years and is becoming a routine...
Comparison of the growth of fast-growing poplar and willow in two sites of Central Kazakhstan
short rotation coppice Salicaceae Central Asia plantation biometric and soil parameters
2017/6/26
In temperate climatic conditions, plantations of poplar and willow species provide sustainable production of biomass. Short rotation coppice plantations on agricultural lands have a great potential to...
Comparison of Carcass Characteristics, Meat Quality, and Blood Parameters of Slow and Fast Grown Female Broiler Chickens Raised in Organic or Conventional Production System
Female Broiler Carcass Quality Meat Quality Organic Feeds
2016/7/27
The objective of the study was to compare the carcass characteristics, meat quality, and blood parameters of slow and fast grown female broiler chickens fed in organic or conventional production syste...
Simple, selective, and sensitive measurement of urea in body fluids of mammals by reversed-phase ultra-fast liquid chromatography
urea derivatization liquid chromatography
2015/6/23
The presented
chromatographic procedure is especially suitable for preparation of reference sample sets, very accurate and
precise research purposes or rapid clinical diagnostic with smaller sampl...
Allometric growth of calcium, phosphorus, magnesium, sodium, and potassium in slow- and fast-growing young chickens
Ca, Mg P, Na K retention
2015/6/23
Allometric coefficients for dry matter, calcium, phosphorus, magnesium, sodium, and potassium in relation
to body weight were 1.091, 1.427, 1.383, 1.284, 0.843, and 1.097 for SG chickens and 1.075, ...
Allometric growth of protein, amino acids, fat and minerals in slow- and fast-growing young chickens
chickens age growth rate chemical allometry amino acid retention
2015/6/9
Allometric growth of body constituents and apparent efficiency of amino acid and nitrogen retention were examined in slow-growing laying-type cockerels (SG) and in fast-growing male broiler hybrids (F...
The meat quality of layer males from free range in comparison with fast growing chickens
breast meat colour fat overall acceptability
2015/6/8
In chick hatcheries, males of laying hybrids are considered to be 搘aste?and the majority of these males are killed just after hatching. On the other hand, the interest of consumers in products from al...
Effects of Housing Systems on Behaviour, Performance and Welfare of Fast-growing Broilers
Broiler Housing System Welfare Behavior Meat Quality
2016/5/16
This experiment aimed to evaluate the effects of different housing systems on behavioral activities, welfare and meat quality of fast-growing broilers. Two hundred broilers were allocated into two hou...
Earlier Metabolizable Energy Intake Level Influences Heat Production during a Following 3-Day Fast in Laying Hens
Net Energy Metabolizable Energy Laying Hens Heat Production Fasting Indirect Calorimetry
2016/5/11
The present study was conducted to estimate energy requirements for maintenance in laying hens by using indirect calorimetry and energy balance. A total of 576 28-wk-old Nongda-3 laying hens with dwar...
Nutrient Use Efficiency of Three Fast Growing Hardwood Species across a Resource Gradient
Nutrient Use Efficiency Resorption N:P Biomass Production
2013/3/4
Attitudes regarding traditional energy sources have shifted toward renewable resources. Specifically, short-rotation woody crop supply systems have become more prevalent for biomass and biofuel produc...
A Stomatal Impression Method Using a Fast-Sticking Adhesive
Arachis hypogaea Peanut Stomatal aperture Stomatal impression
2009/8/4
A Stomatal Impression Method Using a Fast-Sticking Adhesive.
Lysine Requirement of Fast Growing Chickens—Effects of Age,Sex,Level of Protein Deposition and Dietary Lysine Efficiency
age growing chicken lysine requirement modelling
2009/7/30
N-balance studies were carried out to assess the lysine requirement of fast growing chickens (Cobb 500) at different sex and age depending on crude protein deposition and efficiency of dietary lysine ...
DEAESepharose Fast Flow分离纯化刺芹侧耳木质素降解酶
Pleurotus eryngii-Co60-7 木质素降解酶 离子交换层析 DEAE-Sepharose TM Fast Flow
2009/7/1
[目的]为Pleurotus eryngii Co607木质素降解酶的分离纯化和综合利用提供试验依据。[方法]采用DEAE-SepharoseTM Fast Flow离子交换介质,分别考察缓冲液pH值、流速和洗脱方式等对刺芹侧耳木质素降解酶分离纯化的影响,确定了最佳分离纯化层析条件。[结果] DEAESepharoseTM Fast Flow分离纯化Pleurotus er...
Effectiveness of fast neutrons irradiation for the stimulation and induction of genetic changes in soybean (Glycine max L. Merrill) genome
stimulation fast neutrons mutagenesis soybean
2008/11/19
Air-dry seeds of soybean cv. Warszawska were irradiated with fast neutrons (Nf) using the U-120 cyclotron (at the Institute of Nuclear Physics in Cracow) at the doses of 500, 1000, 1500 R. Additionall...