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Heterologous Expression of Xylanase II from Aspergillus usamii in Pichia pastoris
Aspergillus usamii characterization expression xylanase Pichia pastoris
2009/6/24
To efficiently produce xylanase for food processing industry, a gene encoding xylanase II (XynII) from Aspergillus usamii has been cloned into the vector pPIC9K and integrated into the genome of Pichi...
Integrative Expression of Glucoamylase Gene in a Brewer’s Yeast Saccharomyces pastorianus Strain
glucoamylase brewer’s yeast diacetyl fermentation
2009/2/23
The recombinant brewer’s yeast Saccharomyces pastorianus strain was constructed by introducing the ilv2::GLA fragment released from pMGI6, carrying glucoamylase gene (GLA) and using the yeast α-acetol...
Spatial and Temporal Expression of Transferrin Gene In the Rat Mammary Gland
rat mammary transferrin in situ hybridization
2008/7/31
The distribution and concentration of transferrin mRNA in the rat mammary gland was analyzed using in situ hybridization, and the results were compared with those obtained for other milk protein mRNA....
Expression of a Cellobiohydrolase cDNA from the White Rot Fungus Irpex lacteus in Aspergillus oryzae
cellobiohydrolase Irpex lacteus heterologous gene expression
2008/4/20
A cDNA (cel2) coding for an exo-type cellobiohydrolase was isolated from the basidiomycete Irpex lacteus strain MC-2. The cel2 cDNA was expressed in a heterologous host Aspergillus oryzae by using an ...
Cloning and Expression of an Oligo-1,6-glucosidase Gene from Arthrobacter globiformis I42 and Biochemical Characterization of the Recombinant Enzyme
oligo-1 6-glucosidase Arthrobacter globiformis dextran
2008/4/20
The gene encoding an oligo-1,6-glucosidase was cloned in terms of walking downstream from the glucodextranase gene of the chromosomal DNA of Arthrobacter globiformis I42. An open reading frame consist...
Cloning and Expression of Genes Encoding Fructosyltransferases from Higher Plants in Food Technology
asparagus fructo-oligosaccharide fructosyltransferase fructosyltransfer Pichia pastoris
2008/4/20
Recently, we have investigated enzymic production of non-digestible oligosaccharides which have several functional activities as “tertiary functional ingredients” of foods. Fructo-oligosaccharides syn...
Cloning, Sequencing and Expression Analysis of an Endo-1,4-β-D-Xylanase Gene from Aspergillus sojae
Aspergillus sojae xylanase arabinoxylan recombinant protein
2008/4/20
A xylanase gene was cloned from the cDNA library of Aspergillus sojae. The nucleotide sequence suggested that the mature enzyme is preceded by a 22 amino acid signal sequence and the molecular mass wa...
Growth Associated Exopolysaccharide Expression in Lactococcus lactis subspecies cremoris Ropy352
ropy Lactococcus lactis exopolysaccharide
2008/4/1
A natural lactococcal isolate, Lactococcus lactis ssp. cremoris Ropy352, has been previously shown to express two phenotypically distinct exopolysaccharides (ropy and mucoid). This natural isolate was...
Short Communication: Lack of Stage-Specific Embryonic Antigen-1 Expression by Bovine Embryos and Primordial Germ Cells
stage-specific embryonic antigen-1 embryos primordial germ cells bovine
2008/3/31
The objective of this study was to determine whether stage-specific embryonic antigen-1, a cellular marker commonly used to identify murine undifferentiated embryonic cells, is also a useful marker fo...
Expression of Ropy and Mucoid Phenotypes in Lactococcus lactis
ropy phenotype mucoid phenotype Lactococcus lactis Lon protease
2008/3/27
Strains of Lactococcus lactis ssp. lactis and Lactococcus lactis ssp. cremoris were cultured under aerobic and anaerobic conditions on plates of whey agar, Elliker agar, and M17L agar at 15, 20, and 3...
The Effect of Mammary Gland Expression of Human Lysozyme on the Properties of Milk from Transgenic Mice
transgenic human lysozyme milk
2008/3/23
Transgenic mice were used as model systems to evaluate the impact of human lysozyme expression in the mammary gland. We previously generated two lines of transgenic mice that express human lysozyme mR...