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目的 探讨多重实时荧光PCR检测方法对川贝母及伪品鉴别的可行性。方法 通过对常见贝母属植物的ITS、psbA-trnH、rbcL和matK基因序列种间变异、遗传距离及系统发育关系分析，选择进化速率快、种间变异大、种内变异小的基因作为靶基因，设计川贝母及伪品贝母的特异性引物和Taqman探针，建立一种多重实时荧光PCR检测方法。通过特异性、灵敏度及混合样本检测与测序方法比较进行方法评估。
基于改良ERIC-PCR技术快速鉴别人参和西洋参
ERIC-PCR 西洋参 人参 DNA条形码
2016/5/27
为建立快速、准确的鉴定人参和西洋参并测定二者在混合物中含量的方法,将ERIC-PCR(Enterobacterial Repetitive Intergenic Consensus-Polymerase chain reaction)引物改良进行温度梯度PCR扩增,分析人参(Panax ginseng C.A.Mey,PG)和西洋参(Panax quinquefolium L.,PQ)电泳条带并寻...
添加扩增内标的PCR方法快速检测食品中的沙门氏菌
沙门氏菌 PCR检测 扩增内标对照 假阴性
2016/5/19
为了建立一种含扩增内标的沙门氏菌PCR检测方法,以细菌16S r RNA为扩增内标对照,以沙门氏菌inv A基因为靶基因设计了一对引物,并优化了PCR反应体系。通过对20种细菌进行PCR检测显示,该方法对沙门氏菌具有良好的特异性。灵敏度实验表明,该检测方法对沙门氏菌纯DNA模板的检测灵敏度为61.1 fg/μL,对沙门氏菌纯培养物的检测灵敏度为2×102 cfu/m L。对人工污染蛋清的检测实验显...
双重PCR检测生鲜猪肉中大肠杆菌O157∶H7和沙门菌方法的建立与应用
双重PCR检测 食源性致病菌 食品安全
2015/11/17
建立生鲜猪肉中大肠杆菌和沙门菌的双重PCR检测方法,并初步调查生鲜猪肉中大肠杆菌和沙门菌的污染情况。方法 以大肠杆菌O157∶H7 rfbE基因和沙门菌invA基因为靶基因设计引物。通过对单个基因PCR和多重基因PCR扩增进行特异性、敏感性试验以及优化反应体系,建立快速检测大肠杆菌和沙门菌的双重PCR法。在郑州市不同地区的综合性超市、冷鲜肉专卖店和农贸市场随机抽检144份生鲜猪肉样品,分别进行了P...
以采后贮藏过程中自然腐败变质的蓝莓果实为材料,采用传统纯培养法和平板划线法对蓝莓果实表面致腐优势菌进行了分离与形态学鉴定,最终确定葡萄孢霉(Botrytis cinema)为主要病原菌。采用基因组DNA提取试剂盒法提取葡萄孢霉的DNA,并筛选具有特异性的引物,通过聚合酶链式反应(PCR)对葡萄孢霉特定的DNA片段进行扩增,得到具有特异性的目的基因,对其进行分子生物学的特异性鉴定,并对其检测条件进行...
江苏大学现代食品检测技术第十三章 PCR基因扩增技术
江苏大学 现代食品检测技术 第十三章 PCR基因扩增技术
2013/10/25
江苏大学现代食品检测技术第十三章 PCR基因扩增技术。
Evidence for wheat, rye, and barley presence in gluten free foods by PCR method – comparison with ELISA method
polymerase chain reaction ELISA wheat rye barley gliadin
2014/2/26
A method of the evidence for the presence of wheat, rye, and barley in gluten free foods, based on the polymerase chain reaction (PCR), was validated. DNA was isolated from foods by chaotropic solid p...
Quantitative Identification of Rice Cultivars by Real-Time PCR
rice Oryza sativa real-time PCR
2009/6/10
Application of a real-time PCR system for the detection of blending and the quantification of the blending ratio of rice was investigated. Quantitative measurement by real-time PCR was performed using...
Detection of pathogenic Yersinia enterocolitica serotype O:3 by biochemical, serological, and PCR methods
Yersinia enterocolitica serotype O:3 PCR virulence genes biochemical methods cultivation DNA
2014/3/4
In this study, the pathogenic Y. enterocolitica of serotype O:3 was monitored. The serotype is widely spread in Europe and has been linked to human yersiniosis. For the detection of pathogenic strains...
The comparison of the methods for the identification of pathogenic serotypes and biotypes of Yersinia enterocolitica: Microbiological methods and PCR
Yersinia enterocolitica pathogenic serotype PCR detection cultivation DNA identification serotype biotype
2014/3/17
In this study, pathogenic strains of Y. enterocolitica were identified by microbiological and PCR methods. The samples were collected from pigs, cattle, poultry, and slaughter houses. Three common tec...