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Enzyme-Linked Immunosorbent Assay for Simultaneous Detection of Two Fungicides Kresoxim-methyl and Trifloxystrobin in Oranges
kresoxim-methyl trifloxystrobin polyclonal antibody ELISA recovery rate
2016/12/9
To assemble an indirect competitive enzyme-linked immunosorbent assay (ELISA) for estimating two strobilurin fungicides at the same time, the hapten was synthesised which contained the common active g...
Microbiological method using Bacillus megaterium with fusidic acid for detection of macrolides in milk
microbiological test antibiotic erytromycin tylosin tilmicosin
2016/2/23
The microbiological method to attain a sensitive detection of macrolides using Bacillus megaterium in agar medium with fusidic acid was designed. To this aim, Mueller-Hinton medium fortified with gluc...
Accessing The Bioavailability Of Phytochemicals In CACO-2 Cell Model And Developing A Sensitive Method For The Detection And Quantification Of These Compounds
Polymethoxyflavones Transport Permeability
2014/10/22
Numerous studies have found certain unmethylated phytochemicals to possess anti-carcinogenic activity; however, they have been associated with poor oral bioavailability which is a major limiting facto...
Analysis of ochratoxin A in malt beverage samples using dispersive liquid–liquid microextraction coupled with liquid chromatography-fluorescence detection
mycotoxin beverage microextraction HPLC
2014/2/24
A simple and economic procedure based on dispersive liquid–liquid microextraction has been applied to extract and pre-concentrate trace levels of ochratoxin A (OTA) in malt beverage prior to analysis ...
Use of chemometric techniques to design a microbiological method for sulfonamide detection in milk
B. subtilis microbial inhibition method bioassay experimental design logistic regression model
2014/2/24
We proposed an experimental design of a microbial bioassay of dichotomous response (positive or negative) using Bacillus subtilis BGA for the detection of sulfonamide residues. In the first stage, the...
Comparison of detection sensitivity of five microbial inhibition tests for the screening of aminoglycoside residues in fortified milk
antibiotic residues microbial screening detection limits
2014/2/25
The assessment of detection sensitivity of five microbial inhibition tests (MITs), STAR (screening test for antibiotic residues) with the test strain Bacillus subtilis BGA, Delvotest® S P-NT, To...
Utilization of DNA microarrays for detection and identification of selected Fusarium species from the Czech Republic
DNA microarrays fluorescent labelling Fusarium oligonucleotide probe PCR
2014/2/26
Fusarium is a serious phytopathogenic fungal genus with producting of many kinds of highly toxic secondary metabolites – mycotoxins. The consumption of Fusarium contaminated food and feed can cause da...
Methods for detection of Bacillus sp., B. cereus, and B. licheniformis in raw milk
cultivation method chromogenic medium genera-specific PCR species-specific PCR
2014/2/26
Totally 75 raw milk samples were analysed with the methods employing the media compared – MYPA, PEMBA, BrillianceTM Bacillus cereus agar, and HiCrome Bacillus agar. The reference method with MYPA seem...
Simultaneous determination of chloride, bromide and iodide in foodstuffs by low pressure ion-exchange chromatography with visible light detection
LPIEC spectrophotometry halide
2014/2/25
An ion-exchange chromatography method with visible light detection was developed for the simultaneous determination of chloride, bromide, and iodide in foodstuffs. They were separated by means of low ...
Rapid detection of dimethyl yellow dye in curry by liquid chromatography-electrospray-tandem mass spectrometry
azo dyes dimethyl yellow curry LC-ESI-/MS/MS
2014/2/27
An accurate and rapid method, was devised for the identification and quantitation of dimethyl yellow dye in curry, based on liquid chromatography-tandem mass spectrometry interfaced with electrospray....
A Simple and Sensitive Polymerase Chain Reaction Method for the Detection of Food-Related Bacteria
food achromopeptidase DNA preparation
2009/7/8
We devised a simple and efficient method of preparing chromosomal DNA from Gram-positive bacteria for use as a template in PCR, which consists of achromopeptidase treatment followed by Chelex 100 trea...
Evaluation of Chromogenic Enzyme Substrate Mediums, Chromocult Coliform Agar（CCA）and XM-G, by Detection of Freeze-, Heat-, High-Pressure-Injured Coliforms, and Coliforms in Food Samples
chromocult coliform agar chromogenic enzyme substrate medium method conventional method
2009/6/18
The two chromogenic enzyme substrate mediums of chromocult coliform agar (CCA) and XM-G were applied to detect freeze-, heat-, and high-pressure-injured coliforms (Enterobacter aerogenes, Enterobacter...
Detection of Wasabi（Wasabia Japonica Matsum.）in Food Products by Using Myrosinase Genes
myrosinase wasabi Wasabia japonica Matsum
2009/5/20
Wasabi (Wasabia japonica Matsum.) is a Japanese traditional spice. Many kinds of processed foods that include wasabi as an ingredient have been developed and produced. Here, we report on the detection...
Detection Assays of Norovirus Genome Using LightCycler®
LightCycler® PCR equipment Norovirus One-Step Real-Time PCR assay
2009/4/8
In 2003, the Ministry of Health, Labour and Welfare in Japan presented the official assay using ABI PRISM 7000® for the detection of Norovirus (NV) genogroups I (GI) and II (GII). We have recently...
Study of Simple and Rapid Detection of Salmonella spp. Using VIDAS Second Report;The New Protocol with Unique Enrichment Broth
Salmonella mini VIDAS Easy SLM
2009/4/8
In our previous report, we evaluated and reported some VIDAS protocols for the detection of Salmonella. In this report, we evaluated the new additional protocol “Easy SLM” with “SX broth”, a new uniqu...