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用乳酸、乳酸钠与Nisin复合保鲜剂对冷却羊肉进行处理,自封袋包装后在4±1℃的环境中贮藏,测其在贮藏期间的菌落总数、挥发性盐基氮、p H和感官评分,用权重对不同配比的保鲜液对冷却羊肉的保鲜效果进行综合评估,并在此基础上进行优化。结果表明:乳酸浓度0.15%、乳酸钠浓度0.3%、乳酸链球菌素浓度为0.05%时保鲜效果最佳,该处理下的样品在贮藏20 d时各项品质指标仍能保持在鲜肉范围内。
Span-20和Tween-80对发酵液中Nisin泡沫分离的影响
泡沫分离 非离子表面活性剂 乳链菌肽
2009/11/13
含0.5 g/L Tween-80发酵液；Tween-80的加入破坏了气泡表面的网状结构，因而泡沫层持气量较高；连续通气时，2种表面活性剂都会引起泡沫层高度降低，但Tween-80会增加泡沫层的稳定性. 当Tween-80浓度达0.5 g/L时，Nisin的收率大幅度提高，原因是分离过程中Nisin失活率减少.
乳链菌肽发酵玉米浆—糖蜜培养基的优化制备（Optimization Corn Steep Liquor—Molasses Fermentation Medium for Nisin）
乳链菌肽 玉米浆 糖蜜 Box-Behnken设计
2009/8/11
采用单因素试验方法对影响乳链菌肽效价的培养基组分进行了考察。结果表明，影响乳链菌肽效价的主要因素为糖蜜、玉米浆、K2HPO4、MgSO4。在此基础上，采用Box-Behnken设计及响应面分析法确定主要影响因子的最佳质量浓度。结果表明，当玉米浆质量浓度为233g/L，糖蜜质量浓度为27g/L，K2HPO4质量浓度为5.0g/L，MgSO4质量浓度为0.1g/L时，乳链菌肽效价为2491.8IU/m...
Searching for Genes of Lactococcus lactis subsp. lactis Encoding the Bacteriocin Nisin using DNA/DNA Hybridisation
Lactococcus lactis subsp .lactis screening nisin gene PCR probe DNA/DNA hybridisation
2014/3/10
n this work, PCR primers P8 and P9 were used for amplification of a 320 bp long PCR product specific to the nisin gene. The PCR product was labelled with digoxigenine during amplification and used as ...
Identification and characterisation of antimicrobial activity of nisin a produced by Lactococcus lactis subsp. lactis LL27
nisin A Lactococcus lactis sublethal injury heating freezing
2014/3/10
In this study, bacteriocin producing lactococcal strains were isolated from Turkish raw milk samples. Among these isolates, LL27 had the highest inhibition activity against Gram-positive indicator str...
Increased Nisin in Cheddar-Type Cheese Prepared with pH Control of the Bulk Starter Culture System
nisin lactic acid bacteria cheese
2008/3/18
Five strains of Lactococcus lactis ssp. lactis and one transconjugant strain of Lactococcus lactis ssp. cremoris, previously shown to be nisin producers, were evaluated for nisin production during gro...
Shelf-Life of Pasteurized Process Cheese Spreads Made from Cheddar Cheese Manufactured with a Nisin-Producing Starter Culture
cheese spreads nisin spoilage shelf-life
2008/3/18
Cheddar cheese made with a nisin-producing starter culture and Cheddar cheese made with a commercially available starter culture were used to manufacture pasteurized process cheese spreads at low and ...