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Quantitative Dissection of Hydrogen Bond-Mediated Proton Transfer in the Ketosteroid Isomerase Active Site
active site environment computational modeling enzyme catalysis protein electrostatics protein semisynthesis
2016/5/25
Hydrogen bond networks are key elements of protein structure and function but have been challenging to study within the complex protein environment. We have carried out in-depth interrogations of the ...
Quantitative,Directional Measurement of Electric Field Heterogeneity Within an Enzyme Active Site
Quantitative Directional Measurement Electric Field Heterogeneity Enzyme Active Site
2016/5/25
Extensive structural studies of enzymes have revealed that biological catalysis occurs within sequestered active site crevices, where reacting substrates are surrounded by an anisotropic and chemicall...
Anomalous Negative Fluorescence Anisotropy in Yellow Fluorescent Protein (YFP 10C):Quantitative Analysis of FRET in YFP Dimers
Alzheimer's disease Amyloid beta 40 peptide Human serum albumin Circular dichroism Dissociation constant alpha-Helix
2016/5/25
Yellow fluorescent protein (YFP) is widely used as a genetically encoded fluorescent marker in biology. In the course of a comprehensive study of this protein, we observed an unusual, negative fluores...
Quantitative Analysis of Supported Membrane Composition Using the NanoSIMS
lipid bilayer SIMS NanoSIMS mixture gradient compositional analysis
2016/5/24
We have improved methods reported earlier [1] for sample preparation, imaging and quantifying components in supported lipid bilayers using high-resolution secondary ion mass spectrometry performed wit...
