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树突状细胞（DC）疫苗是目前在临床上应用的一种肿瘤治疗疫苗，其制备过程一般都在病人体外进行，病人来源的DC在体外进行诱导分化后再回输给病人，这种疫苗制备过程十分复杂，容易被污染，不同的技术人员、不同条件的实验室制备的疫苗疗效差异较大，较难标准化，因此体外制备的DC疫苗很难在临床推广应用。
近日，免疫学专业期刊《白细胞生物学杂志》（Journal of Leukocyte Biology）在线发表题为《鼻腔上皮细胞通过TLR5/GM-CSF调节呼吸道DC并增强IgA应答》（Nasal epithelial GM-CSF contributes to TLR5-mediated modulation of airway dendritic cells and subsequent Ig...
A new record for Turkey: Michauxia nuda A.DC. (Campanulaceae)
Campanulaceae Michauxia new record flora Turkey
2010/3/17
Michauxia nuda A.DC. (Campanulaceae) is recorded for the first time (C8 Siirt province) from Turkey. The diagnostic characters of M. nuda are described and its habitat and conservation status are indi...
Pollen morphology of Astragalus L. sect. Onobrychoidei DC. (Fabaceae) in Turkey
Turkey Fabaceae Astragalus Onobrychoidei pollen morphology
2010/3/17
The pollen morphology of 45 specimens of 29 species of the genus Astragalus L. of the section Onobrychoidei DC. distributed in Turkey was studied with light and electron microscopies. The pollen is ge...
中国狗牙花属[Ervatamia (A.DC.) Stapf]的研究
研究 狗牙花属 中国
2009/7/14
中国狗牙花属[Ervatamia (A.DC.) Stapf]的研究。
大花甘松（Nardostachys grandiflora Dc.)和中华甘松（N.Chinensis Batalin)的精油成分及其在香料上的应用研究
中花 中华 甘松 成分 应用
2008/3/20
本文报道滇产大花甘松和蜀产中华甘松精油的化学成分。利用气相色谱、光谱数据、化学降解和衍生物的制备，从大花甘松精油中鉴定了五种成分：甘松醇（I）（69.4％），△9,10-马兜铃烯（Ⅶ）（0.2％），△1, 10一马兜铃烯（Ⅷ）（2％），β-橄榄烯（Ⅸ）(2.9％）和1，2，9，10-四去氢马兜铃烯（Ⅳ）（2.9％）。中华松精油中上述五种成分的含量分别为：I（8％），Ⅶ（2.1％），Ⅷ（8.6％），...
以双脱氧链终止法测定dG、dC接尾的cDNA克隆序列
双脱氧链终止法 dG、dC接尾 cDNA克隆序列
2008/1/17
组建重组克隆常采用均聚加尾法，即以dG尾加在经Psi I酶切的质粒载体上，dC尾加在待克隆的双链。DNA分子上，这样产生的带有均聚尾的重组克隆给双脱氧链终止法［2, 3]侧定序列带来很大的困难，即接尾后的序列往柱不能阅读。我们对序列测定的某些步骤进行了修改，使接尾后的序列清晰可读。
摘要 在完成小花棘豆毒素 95 %氨基酸序列的基础上 ,根椐已知的氨基酸序列 ,设计合成了特异简并引物 .以小花棘豆总RNA为模板 ,逆转录合成cDNA第一链 ,用置换法合成双链cDNA .用特异引物对此双链cDNA进行PCR扩增 ,将扩增后的目的基因与用SmaⅠ酶切的质粒pUC 18连接 ,转化大肠杆菌JM10 7.筛选阳性克隆进行序列分析 ,获得了OXY基因的全部序列 .经测序后测得基因序列与...
摘要 纳豆激酶是一种纤维蛋白溶解酶 ,有望开发成为新型的溶栓药物 .从中国豆豉中分离的具有较强纤溶活性的枯草杆菌DC 2中提取总DNA ,根据纳豆激酶 (NK)基因序列设计引物 ,用PCR法扩增NK基因 .序列分析表明 ,NK基因成熟肽编码区含有 82 5bp ,编码 2 75个氨基酸残基 ,与文献报道的序列分别有 93 4 %和 94 5 %同源性 .将NK基因插入载体pGEX 4T1构建表达...
A New Record for Turkey: Malcolmia exacoides (DC.) Spreng. (Brassicaceae)
Brassicaceae Malcolmia new record Turkey
2010/3/18
Malcolmia exacoides (DC.) Spreng. (Brassicaceae) is recorded for the first time (C9 Siirt) for the flora of Turkey. The diagnostic characters of M. exacoides are given and its ecology and conservation...
A New Genus (Leptaleum DC.) Record for Turkey
Flora of Turkey Kars Cruciferae/Brassicaceae Leptaleum new record
2010/3/18
e monotypic Leptaleum DC. (Cruciferae/Brassicaceae) is recorded for the first time from Turkey. The diagnostic characters of Leptaleum filifolium (Willd.) DC. are given and its ecology and conservatio...
Serratula oligocephala DC. (Asteraceae), a New Record for the Flora of Lebanon
Biodiversity endemic flora Mount Lebanon Mediterranean
2010/3/18
Serratula oligocephala DC. is currently reported as an endemic species of Turkey, where it has limited distribution in east and south Anatolia. Specimens collected from the continental humid slopes be...
Two New Records of Astragalus Species of the Sections Anthylloidei DC. and Dissitiflori DC. from Iran
Anthylloidei Astragalus A. crassispinus A. nigrolineatus Cercidothrix Dissitiflori Fabaceae Iran
2010/3/19
Astragalus crassispinus Bunge and Astragalus nigrolineatus Sirj. & Rech.f. are new records from Iran. They belong to the sections Anthylloidei DC. and Dissitiflori DC. These species were endemic to Af...
A New Record From Turkey: Jurinea stoechadifolia (M.Bieb) DC.
Jurinea new record Turkey Compositae
2010/3/23
Jurinea stoechadifolia (M.Bieb) DC. ( Compositae) is reported for the first time (A4 Çankiri) for the flora of Turkey.
A New Taxon for Turkey: Galium recurvum Req. ex DC. ( Rubiaceae)
Rubiaceae Galium recurvum new record Turkish flora
2010/3/23
It is reported here that Galium recurvum Req. ex DC., collected from Ayvalik (province of Balikesir), is new to the Turkish flora. In this article a photograph, a short morphological description, incl...