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Characterization of two second messenger pathways and their interactions in eliciting the human sperm acrosome reaction
Signal transduction protein kinase A protein kinase C crosstalk acrosome reaction
2009/2/13
The human sperm acrosome reaction (AR) occurs via the activation of at least two signal transduction pathways. The purpose of this investigation was to characterize two of the pathways, the protei...
Interleukin-6 enhances the fertilizing capacity of human sperm by increasing capacitation and acrosome reaction
Interleukin-6 enhances human sperm acrosome reaction
2009/2/12
The effects of human recombinant interleukin-6 (IL-6) on human sperm penetration of zona-free hamster ova (SPA) and human sperm cell capacitation and acrosome reaction were investigated. IL-6 at h...
Atrial natriuretic peptide(ANP)as a stimulus of the human acrosome reaction and a component of ovarian follicular fluid:correlation of follicular ANP content with in vitro fertilization outcome
Noncapacitated spermatozoa capacitation
2009/2/12
Atrial natriuretic peptides (ANPs) from several species induced the human acrosome reaction. The maximal response was highest for human ANP (18.6% above unstimulated or baseline values) and decrea...
The acrosome reaction-inducing effect of human follicular and oviductal fluid
reaction-inducing effect human follicular oviductal fluid
2009/2/12
The human sperm acrosome reaction (AR) can be induced in vitro by a variety of naturally occurring and synthetic compounds. The present investigation determined the effect of human natural cycle per...
Analysis of the responses of human spermatozoa to A23187 employing a novel technique for assessing the acrosome reaction
A231 87 acrosome reaction oocyte fusion human
2009/2/12
Protocols for the use of A23187 in assessing the ability of human spermatozoa to acrosome-react and exhibit sperm-oocyte fusion have been developed and the results compared in two independent cohort...
The human sperm acrosome reaction does not depend on arachidonic acid metabolism via the cyclooxygenase and lipoxygenase pathways
human spermatozoa acrosome reaction arachidonic acid eicosanoids cyclooxygenase lipoxygenase
2009/2/11
The objective of this study was to determine whether the metabolism of arachidonic acid via the cyclooxygenase pathway, the lipoxygenase pathway, or both has a pivotal role in the human sperm acro...
Facilitative effect of pulsed addition of dibutyryl cAMP on the acrosome reaction of noncapacitated human spermatozoa
pulsed addition dibutyryl cAMP noncapacitated human spermatozoa
2009/2/11
The in vitro acrosome reaction of noncapacitated human spermatozoa was induced by both calcium ionophore (A23187) and dibutyryl adenosine cyclic monophosphate (Bu2cAMP), a membrane permeant cyclic n...
A test of the human sperm acrosome reaction following ionophore challenge. Relationship to fertility and other seminal parameters
zation infertility human spermatozoa calcium
2009/2/7
Acrosome reaction capacity was tested on semen samples from 53 fertile and 26 subfertile men. Preparations were divided into two aliquots after 3 or 24 hours of culture. One aliquot received 10 mumo...
Effect of egg yolk medium on the acrosome reaction of human spermatozoa
human spermatozoa capacltation acrosome reaction egg yolk medium
2009/2/6
Preincubation of human spermatozoa in an egg yolk medium (TESTY) at 5 C, followed by washing at 37 C by centrifugation and resuspension in a standard medium (BWW), enhanced the percentage of spermat...
Capacitation and Induction of the Acrosome Reaction in Bull Spermatozoa With Norepinephrine
Catecholamines bovine
2008/12/30
Identification of norepinephrine (NE) within the microenvironment of the bovine oviduct suggests a potential role for catecholamines in the events surrounding fertilization. Previous studies have s...
Detection of the mouse acrosome reaction by acid phosphatase Comparison with chlortetracycline and electron microscopy
mouse acid phosphatase chlortetracycline electron microscopy
2008/12/26
The sperm acrosome is a uniquely regulated secretory vesicle containing several hydrolase enzymes, including acid phosphatase (AP). The exocytotic event that releases these enzymes, the acrosome react...
Actin and actin-binding proteins in bovine spermatozoa:potential role in membrane remodeling and intracellular signaling during epididymal maturation and the acrosome reaction
proteins bovine spermatozoa potential role signaling epididymal maturation
2008/12/26
The actin cytoskeleton influences a wide range of functions in nonmuscle somatic cells, including shape, movement, and interactions with extracellular matrices. The role of actin in mammalian male ger...
The cyclic GMP-specific phosphodiesterase inhibitor, sildenafil, stimulates human sperm motility and capacitation but not acrosome reaction
cyclic GMP-specific phosphodiesterase inhibitor sildenafil human sperm motility capacitation
2008/12/25
Capacitation is the series of transformations that spermatozoa undergo in the female genital tract in order to bind to the zona pellucida, initiate the acrosome reaction, and fertilize an egg. Cyc...
Cholesterol inhibitory effects on human sperm-induced acrosome reaction
Cholesterol inhibitory human sperm-induced acrosome reaction
2008/12/25
Progesterone (P4) is known to induce an acrosome reaction in mammalian sperm in vitro, whereas cholesterol is a major inhibitor of acrosome reaction. This study had three objectives: to study the in...
Effects of accessory sex gland fluid on viability, capacitation, and the acrosome reaction of cauda epididymal bull spermatozoa
sex gland viability capacitation reaction bull spermatozoa
2008/12/25
The effect of accessory sex gland fluid (AGF) on viability and acrosomal integrity of spermatozoa was examined with cauda epididymal spermatozoa and AGF from the same Holstein bull (n = 6). Surgical...