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Effect of dilauroylphosphatidylcholine on human spermatozoa
dilauroylphosphatidylcholine human spermatozoa
2009/2/10
To achieve higher rates of acrosomal loss for in vitro studies of human sperm function, the effect of liposomes prepared from the phospholipid, dilauroylphosphatidylcholine (PC12), on human sperma...
A comparison of critical osmolality and hydraulic conductivity and its activation energy in fowl and bull spermatozoa
Water permeability plasma membrane flow cytometry
2009/2/10
Measurements were made of critical osmolality, the osmolality at which 50% of the cells are lysed, and of the permeation time, the time taken to lyse 50% of the cells in an osmotic solution lower ...
Biochemical alterations in the proacrosin-acrosin system during epididymal maturation of the rat spermatozoa
Biochemical alterations proacrosin-acrosin system epididymal maturation rat spermatozoa
2009/2/10
Acrosin, an acrosomal serine protease, is believed to have a role in fertilization. The enzyme is synthesized in an enzymatically inactive precursor form, proacrosin, and is processed to enzymatical...
Intracellular calcium accumulation and responsiveness to progesterone in capacitating human spermatozoa
Spermatozoa [Ca2+]i progesterone capacitation
2009/2/7
Progesterone induced a rapid, long-lasting, dose-dependent increase of intracellular free calcium concentration ([Ca2+]i) in human sperm capacitated overnight. This effect was not counteracted by th...
The fix vital stain method. Simultaneous determination of viability and acrosomal status of bovine spermatozoa
Acrosomal evaluation vital staining Hoechst bisbenzimide
2009/2/7
A rapid, accurate and precise method for simultaneous determination of acrosomal status and viability of bull spermatozoa is introduced and evaluated. The method involves fixation of semen with glut...
Relationship between morphology and motion characteristics of human spermatozoa in semen and in the swim-up sperm fractions
sperm morphology motility patterns swim-up separation
2009/2/6
In this study, the authors evaluated the morphology pattern and motion characteristics of human spermatozoa before and after swim-up separation. Samples were divided into two, morphologically diff...
Effect of egg yolk medium on the acrosome reaction of human spermatozoa
human spermatozoa capacltation acrosome reaction egg yolk medium
2009/2/6
Preincubation of human spermatozoa in an egg yolk medium (TESTY) at 5 C, followed by washing at 37 C by centrifugation and resuspension in a standard medium (BWW), enhanced the percentage of spermat...
Kinematics of human spermatozoa incubated under capacitating conditions
motility hyperactivation videomicrography
2009/2/6
Suspensions of seminal plasma-free human spermatozoa were prepared by swim-up from semen and studied using high magnification videomicrography after incubation under capacitating conditions for 1.5-...
Characterization of sulfhydryl proteins involved in the maintenance of flagellar straightness in hamster spermatozoa
sperm flagella motility sulfhydryl proteins
2009/2/6
Hamster caput epididymal spermatozoa exhibit a marked 90-180 degree bend when induced to acquire progressive motility in vitro (Cornwall et al, 1988). Flagellar bending is prevented by oxidizing spe...
Sialyglycoconjugates release during in vitro capacitation of human spermatozoa
human sperm capacitation glycoconjugates
2009/2/6
Modifications of the human sperm surface during incubation in capacitating conditions were studied by radiolabeling terminal sialic acid residues of cell surface glycoconjugates using the sodium met...
Addition of hypotaurine can reactivate immotile golden hamster spermatozoa
hypotaurine epinephrine penicillamine
2009/2/6
Hamster epididymal spermatozoa became virtually immotile following washing and dilution in chemically defined medium (TLP-PVA). The sperm motility factors (penicillamine, hypotaurine, and epinephrin...
Capacitation and Induction of the Acrosome Reaction in Bull Spermatozoa With Norepinephrine
Catecholamines bovine
2008/12/30
Identification of norepinephrine (NE) within the microenvironment of the bovine oviduct suggests a potential role for catecholamines in the events surrounding fertilization. Previous studies have s...
Enolase isoforms activities in spermatozoa from men with normospermia and abnormospermia
Human sperm glycolytic enzyme Percoll gradient enzyme marker sperm immaturity
2008/12/30
Total enolase as well as enolase-alphaalpha. (ENO-alphaalpha, ubiquitous) and enolase-S (ENO-S, sperm-specific) activities were measured in total and Percoll-selected sperm from 30 normospermic fert...
Effect of different incubation conditions on phosphatidylserine externalization and motion parameters of purified fractions of highly motile human spermatozoa
Motility incubation phosphatidylserine translocation
2008/12/30
The objective of this study was to assess the temporal effects of sperm incubation at body temperature with various amounts of human serum albumin (HSA) on motion parameters and phosphatidylserine e...
Osmotic tolerance of equine spermatozoa and the effects of soluble cryoprotectants on equine sperm motility, viability, and mitochondrial membrane potential
Sperm osmotic stress horse cryopreservation glycerol
2008/12/29
Osmotic stress attributed to differences in the relative permeability of cryoprotectants, such as glycerol and water, appears to be an important factor in cryodamage. The objective of this study was...