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The Effect of Xylene and Formaldehyde Inhalation on Testicular Tissue in Rats
Testes Relaxin-like Factor Xylene Formaldehyde Rat
2016/5/5
In this study, changes in testicular tissues of rats subjected to xylene and formaldehyde inhalation were evaluated. Three experimental groups were included in the study. Each group of rats was expose...
Validation of 17 Microsatellite Markers for Parentage Verification and Identity Test in Chinese Holstein Cattle
Parentage Analysis Identity Test Microsatellite Multiplex PCR Chinese Holstein
2016/5/4
To develop an efficient DNA typing system for Chinese Holstein cattle, 17 microsatellites, which were amplified in four fluorescent multiplex reactions and genotyped by two capillary electrophoresis i...
Molecular Cloning, Tissue Distribution and Expression of Porcine y+L Amino Acid Transporter-1
Cloning Pig Amino Acid Transporter y+LAT1 SLC7A7
2016/5/4
In this study, we cloned, sequenced and characterized porcine y+L Amino Acid Transporter-1 (y+LAT1). By screening a translated EST database with the protein sequence of the human y+LAT1 and by using r...
成体雌性小熊猫卵巢组织学观察
小熊猫 组织学 卵巢 繁殖
2016/7/20
2000 年至2009 年,12 只固定于10% 福尔马林中非生殖系统疾病死亡的小熊猫卵巢组织,按常规组织学技术制作组织切片,HE 染色,光学显微镜观察。结果:(1)不同发情时期卵巢均有原始卵泡、初级卵泡和次级卵泡分布。发情期的卵巢未观察到典型的成熟卵泡和卵母细胞; (2)原始卵泡数量较少,初级卵泡数量较多,多数初级卵泡和大多数的次级卵泡都处在闭锁状态;(3)卵泡腔出现之前,卵母细胞的直径和卵泡直...
高原鼠兔脑红蛋白基因的克隆与组织表达
脑红蛋白 高原鼠兔 基因克隆 基因表达
2016/7/20
克隆高原鼠兔脑红蛋白(Neuroglobin,NGB)基因编码区并检测其在成年高原鼠兔脑组织和其他组织中的表达,同时探讨高原鼠兔低氧适应的分子生物学机制。从高原鼠兔脑组织中提取总RNA,通过RT - PCR 获得高原鼠兔NGB cDNA,将其与pGEM - T Easy 载体连接,构建重组质粒,蓝白斑筛选阳性克隆并进行鉴定和测序;制备地高辛标记的RNA 探针并采用原位杂交法(In suit hyb...
废旧华太电池液对涡虫的毒性作用
日本三角涡虫 华太电池 生存
2009/11/10
［目的］探讨不同浓度电池液对涡虫的毒害作用。［方法］用不同浓度、不同型号的废旧华太电池液对日本三角涡虫进行饲养，观察涡虫的生存情况及再生情况。［结果］试验结果表明，随着废旧华太电池液稀释倍数的减小，对涡虫的毒害作用增大，涡虫死亡或解体数增多；废旧华太电池液稀释一定倍数后虽然不能使涡虫死亡或解体，但影响涡虫的再生情况。随着废旧华太电池液稀释倍数的减小，涡虫的再生速度明显减慢，甚至无法进行再生。［结论...
保存温度与时间对动物组织DNA提取质量的影响
三疣梭子蟹 肌肉组织 DNA提取 保存条件
2009/12/30
［目的］研究不同保存温度、不同保存时间对蟹肉总DNA提取质量的影响，为同类研究提供借鉴。［方法］将样品在4、-20和-40 ℃下分别保存4、8和15 d，提取螯足内肌肉总DNA，测定总DNA的紫外吸收值，并设计引物扩增其mtDNA ND6基因，采用琼脂糖凝胶电泳分别对提取的总DNA及PCR结果进行检测。［结果］由鲜活蟹组织提取的DNA A260与A280的比值因蛋白污染...
金丝猴(Rhinopithecus)的臂神经丛
臂神经丛 金丝猴
2009/6/23
本文为首次报导三种金丝猴的臂丛观察。结果表明：金丝猴的臂丛由C[(4)5]-Th[1]神经根组成，并不包含多数猴科动物所具有的Th[2]，属于前置型。和人类不同，金丝猴的臂丛虽结合为三干三索，但是代表后索的仅是两个神经袢，并且由神经袢直接发出肩胛下神经、腋神经和桡神经。金丝猴和疣猴一样都存在大圆肌神经；和叶猴一样都有两条胸前外侧神经，而且胸背神经都发自桡神经。其它神经与人类相似。
本文应用光镜、电镜技术研究了仿刺参(Apostichopus japonicus)体腔细胞和血窦中血细胞的形态、结构、周年体腔细胞总数及主要类型的细胞数量。研究结果表明，仿刺参体腔细胞和血细胞形态结构相同，作者将体腔细胞分为淋巴样细胞、球形细胞、变形细胞、透明细胞、纺锤细胞和结晶细胞，其中球形细胞又分为Ⅰ型、Ⅱ型、Ⅲ型。血窦中血细胞除未见结晶细胞外，其余与体腔细胞相同。体腔细胞和血细胞均以淋巴样细...
Cloning of OLR1 Gene in Pig Adipose Tissue and Preliminary Study on Its Lipid-accumulating Effect
OLR1 Pig Lipid Metabolism Adipose Tissue
2016/5/3
In this study we cloned and characterized a novel lipid-accumulating gene, the oxidized low-density lipoprotein receptor 1 (OLR1), which is associated with lipogenesis. We analyzed the gene structure ...
Supplementation Effects of C18:2 or C18:3 Rich-oils on Formations of CLA and TVA, and Lipogenesis in Adipose Tissues of Sheep
Conjugated Linoleic Acid Oils Lipogenesis Lipogenic Enzymes Adipose Tissue Sheep
2016/5/3
The present study was conducted to investigate the supplementation effects of C18:2 rich-soybean oil or C18:3 rich-perilla oil (7% of total diet, DM basis) for 12 weeks on plasma metabolites, fatty ac...
Melanocyte-stimulating Hormone Receptor (MC1R) Genotype and Its Effects on Coat Color in Korean Jindo Dogs
Single Nucleotide Polymorphisms Microsatellite Markers Coat Color
2016/4/29
The Jindo dog is a Korean natural monument and is recognized by the Fédération Cynologique Internationale. A prominent feature is the diverse coat color within the breed. To analyze the genetic basis ...
Effect of Chitosan on Nitric Oxide Content and Inducible Nitric Oxide Synthase Activity in Serum and Expression of Inducible Nitric Oxide Synthase mRNA in Small Intestine of Broiler Chickens
Chitosan Broiler Chickens Nitric Oxide Inducible Nitric Oxide Synthase mRNA Expression
2016/4/29
The present study was conducted to determine the effects of chitosan on nitric oxide (NO) content and inducible nitric oxide synthase (iNOS) activity in serum, and relative expression of iNOS mRNA in ...
In vivo Methane Production from Formic and Acetic Acids in the Gastrointestinal Tract of White Roman Geese
Acetic Acid Formic Acid Gastrointestinal Tract Geese Methane
2016/4/29
Three experiments were conducted to determine the conversion rate of formic and acetic acids into methane in the gastrointestinal tracts of geese. In experiment I, two sets of two 4-month-old male Whi...
Proteomic Approach Analysis of Mammary Membrane Proteins Expression Profiles in Holstein Cows
Holstein Cows Mammary Gland Proteome Bioinformatics Software
2016/4/29
To investigate host defense mechanisms for protecting the mammary gland from mastitis infection, the membrane fraction of mammary tissues from Holstein cows was purified by differential velocity centr...