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为确定引起枸杞霉变的菌种类型,该文以采后鲜枸杞果实为材料,分离导致其霉变的菌种,经形态学特征及分子生物学特征鉴定确定类型,并对与其霉变速率相关的部分生物学特性进行研究。结果表明:从霉变的鲜枸杞中共分离出6株致霉菌,分别鉴定为:菌种1为镰刀菌、菌种2为交链孢霉、菌种6为尖孢镰刀菌、菌种7为青霉菌、菌种11为串珠状赤霉菌及菌种21为草酸青霉。经过回接试验发现6种霉菌均能使健康枸杞鲜果霉变,从这些病斑中...
旨在了解梅花鹿鹿茸不同部位蛋白表达差异,本研究以腊片部位、血片部位和骨片部位的梅花鹿鹿茸组织为试验材料,运用双向电泳(2-DE)和MALDI-TOF-TOF串联质谱技术对不同部位梅花鹿鹿茸差异表达蛋白质进行鉴定,并对差异蛋白进行生物信息学分析。结果表明,成功筛选出37个差异表达蛋白,主要涉及多细胞生物体发生、解毒、细胞成分组织或生物发生、细胞聚集、定位、对刺激的反应、生物黏附、发育、单一生物体发生...
Genome-wide polymorphisms show unexpected targets of natural selection
population genomics natural selection
2015/12/11
Natural selection can act on all the expressed genes of an individual, leaving signatures of genetic differeniation or diversity at many loci across the genome. New power to assay these genome-wide ef...
4.0-A resolution cryo-EM structure of the mammalian chaperonin TRiC/CCT reveals its unique subunit arrangement
4.0-A resolution cryo-EM structure mammalian chaperonin TRiC/CCT reveals unique subunit arrangement
2015/12/11
The essential double-ring eukaryotic chaperonin TRiC/CCT (TCP1-ring complex or chaperonin containing TCP1) assists the folding of approximately 5-10% of the cellular proteome. Many TRiC substrates can...
Interplay between chaperones and protein disorder promotes the evolution of protein networks
Interplay between chaperones protein disorder promotes evolution protein networks
2015/12/11
Evolution is driven by mutations, which lead to new protein functions but come at a cost to protein stability. Non-conservative substitutions are of interest in this regard because they may most profo...
A Direct Regulatory Interaction between Chaperonin TRiC and Stress-Responsive Transcription Factor HSF1
Direct Regulatory Interaction TRiC Stress-Responsive Transcription Factor HSF1
2015/12/11
Heat shock transcription factor 1 (HSF1) is an evolutionarily conserved transcription factor that protects cells from protein-misfolding-induced stress and apoptosis. The mechanisms by which cytosolic...
An Application for Downloading and Integrating Molecular Biology Data
downloading molecular biology data integrating data
2015/5/26
Integrating large volumes of data from diverse sources is a formidable challenge for many investigators in the field of molecular biology. Developing efficient methods for accessing and integrating th...
利用16S rDNA基因及线粒体DNA细胞色素氧化酶亚基Ⅰ(cytochrome oxidase subunit I gene,COⅠ)基因建立3种形态学特征相近的血蜱属蜱类-长角血蜱、褐黄血蜱及铃头血蜱的分子生物学鉴定方法,探讨它们的系统发生关系。 方法 在上海市动物体表采集寄生蜱,在解剖显微镜下进行形态学鉴定后,提取蜱样本基因组DNA,采用PCR方法从蜱基因组中扩增16S rDNA及COⅠ基因...
Changes in quality characteristics of Golden Delicious apples under different storage conditions and correlations between them
apples Golden Delicious cv. storage fruit analyses
2015/1/20
In this four-year study, fruits of Golden Delicious cv. randomly sampled from four different orchards on M 9 were kept at 1 or 2oC in air storage, and in the course of storing individually assessed fo...
An Application for Downloading and Integrating Molecular Biology Data
downloading molecular biology data integrating data
2015/1/9
INTRODUCTION AND BACKGROUND Integrating large volumes of data from diverse sources is a formidable challenge for many investigators in the field of molecular biology. Developing efficient methods for ...
Molecular Biology of a Surface-Exposed Protein Family of Bartonella Bacilliformis
Bartonella Bacilliformi Surface-Exposed Protein
2015/1/7
Bartonella bacilliformis (Bb) is the bacterial agent of Carrión's disease and is presumed to be transmitted between humans by phlebotomine sand flies, most notably Lutzomyia verrucarum. Carrión's ...
A Molecular Phylogenetic Assessment of Pseudendoclonium
taxonomy green algae Molecular Phylogenetic Assessment
2015/1/6
ABSTRACT A MOLECULAR PHYLOGENETIC ASSESSMENT OF PSEUDENDOCLONIUM SEPTEMBER 2007 RICHARD F. MULLINS JR., B.S., UNIVERSITY OF MASSACHUSETTS AMHERST Directed by: Professor Daniel R. Cooley Pseudendocloni...
Volatile Profiles and Resistance to Herbivory in Eastern Hemlock
plant-herbivore interactions host resistance
2015/1/4
Eastern hemlock hosts the hemlock woolly adelgid, an introduced sap-feeding insect that causes rapid deterioration of the host. Like most conifers, eastern hemlock produces a variety of constitutive a...
Expression and Purification of Human Lysosomal β-galactosidase from Pichia Pastoris
human β-galactosidase lysosomal storage diseases Pichia pastoris protein expression protein purification biochemistry and molecular biology
2014/12/12
Lysosomal storage diseases are genetically inherited diseases caused by the dysfunction of lysosomal enzymes. In a normal cell, lysosomal enzymes cleave specific macromolecules as they are transported...
Proteolytic Regulation of CtrA, the Master Regulator of Cell Cycle in Caulobacter crescentus
Caulobacter proteolysis CtrA SciP
2014/12/9
Cell cycle progression in Caulobacter crescentus depends on the master regulator, CtrA. During the transition from swarmer to stalk cell (G1 to S phase), CtrA is degraded by the AAA+ protease ClpXP an...