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CRISPR-Cas12a (formerly Cpf1) is an RNA-guided endonuclease with distinct features that have expanded genome editing capabilities. Cas12a-mediated genome editing is temperature sensitive in plants, bu...
The vegetation coverage is one of the important factors that restrict the accuracy of remote sensing retrieval of soil moisture. In order to effectively improve the accuracy of the remote sensing re...
The aim of this study was to verify the high resolution melting (HRM) method in the analysis of single nucleotide polymorphism (SNP) and simple sequence repeat (SSR) markers in pea (Pisum sativum L.)....
To study the effect of exogenous cytokinin application on wheat yield, an experiment was conducted in 2005 - 2006. Three varieties of wheat (Azadi cross, Darab 2 and Niknezhad) and four levels of BAP ...
Characteristics of a multiline variety composed of blast resistance isogenic lines “Koshihikari BLs”, bred in Niigata, were evaluated and compared with those of the original variety, Koshihikari for...
摘要单核苷酸多态性(single nucleotide polymorphism,SNP)是等位基因间序列差异最为普遍的类型,可作为一种高通量的遗传标记。已建立了PCR扩增目标序列及其产物测序和电子SNP(eSNP)等多种发现和检测SNP的方法。玉米和大豆等作物也已开展了SNP分析。一些栽培作物种质的多样性不断减少,其结果使连锁不平衡(linkage disequilibrium,LD)增加,这有...
摘要 野生稻由于长期处于野生状态,经受了各种灾害和不良环境的自然选择,抗逆性较强,是天然的基因库,保存着栽培稻不具有或已经消失了的特异基因。挖掘野生稻特异基因的研究日益受到重视。本文总结了近3年来在野生稻特异性状基因定位、克隆和育种利用方面的研究进展。 Abstract:Wild rice has adapted to weather and unfavorable environments u...
本研究将除模板DNA以外的PCR反应混合液,经过真空浓缩冻成干粉状后,分别置于室温和4℃贮存,经过一定的贮藏时间后进行PCR反应,发现经冻干处理的样品能在较长的贮存时间内保持扩增活性,这将给实际工作带来很大的便利。

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