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Extraction of CSVd-RNA by Boiling for Dot Blot Hybridization
Hybridization CSVd-RNA Extraction
2009/7/13
A new and simple boiling method for extracting chrysanthemum stunt viroid (CSVd) RnA is described. Fresh leaves of CSVd-infected chrysanthemum were homogenized in an equal volume of CTAB buffer then c...
Dot-blot assay for the semiquantitative detection of major dust mite allergens Derp1 and Derf1 in house dust
dot blot enzyme-linked immunosorbent assay house dust mite mite allergen
2010/11/25
Mite allergens Derp1 and Derf1 were tested in 200 house dust samples using a nitrocellulose membrane-based dot-blot assay. The assay enables the mite allergens in a dust extract to be detected with re...
