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An improved mouse sperm-oocyte plasmalemma binding assay: studies on characteristics of sperm binding in medium with or without glucose
Zona-free oocytes pronuclear zygotes in vitro fertilization
2009/2/18
Sperm binding to the oocyte plasmalemma is crucial to subsequent steps in fertilization. However, the usual in vitro assay for sperm-oocyte binding does not distinguish between nonspecific attachmen...
The impact of reactive oxygen species on bovine sperm fertilizing ability and oocyte maturation
Spermatozoa fertilization development xanthine oxidase
2009/2/17
The objective of this study was to examine the effects of reactive oxygen species (ROS) on bovine sperm function and on the developmental competence of in vitro-matured bovine oocytes. In a first se...
Evaluation of human sperm hyperactivated motility and its relationship with the zona-free hamster oocyte sperm penetration assay
Motility capacitation fertilizing capacity
2009/2/7
The authors studied hyperactivated motility of human spermatozoa as a method of evaluating capacitation by examining its relationship to results of zona-free hamster oocyte sperm penetration assays ...
A Retrospective Comparison of Pregnancy Outcome Following Conventional Oocyte Insemination vs Intracytoplasmic Sperm Injection for Isolated Abnormalities in Sperm Morphology Using Strict Criteria
In vitro fertilization zona pellucida subfertile male
2008/12/24
Initial in vitro fertilization (IVF)–embryo transfer studies found poor fertilization and pregnancy rates following conventional fertilization of oocytes when using sperm with <4% normal morpholo...
Successful Pregnancy and Delivery Following Intracytoplasmic Injection of Frozen-Thawed Nonviable Testicular Sperm and Oocyte Activation With Calcium Ionophore
Successful Pregnancy Delivery Intracytoplasmic Injection Frozen-Thawed Nonviable Testicular Sperm Oocyte Activation
2008/12/24
Several pregnancies have been reported using frozenthawed testicular sperm (Fisher et al, 1996; Podsiadly et al, 1996; Khalifeh et al, 1997), stressing the role of sperm motility for successful outc...
Induction of the acrosome reaction and zona-free hamster oocyte penetration by a bull with complete teratospermia versus a half brother with normal sperm
zona-free hamster oocyte penetration bull complete teratospermia
2008/12/1
A fertile bull producing normal sperm and a sterile half brother exhibiting 100% teratospermia were available to study an induced sperm acrosome reaction and oocyte penetration. Pedigree analysis in...