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A synchronous acrosome reaction system was established for human spermatozoa. Seminal plasma is removed from the spermatozoa by centrifugation and the washed spermatozoa are capacitated in a modif...
It should be recalled that sperm capacitation was originally defined in 1952 as some physiological changes of the spermatozoa in the female genital tract before they are capable of penetrating and fer...
The temporal involvement of Ca2+ during mouse sperm capacitation in vitro was examined by releasing sperm into Ca2+-free medium and then introducing Ca2+ (1.80 mM, final concentration) at 30 minute ...
The objective was to obtain in vitro sperm capacitation and fertilization to approximate the normal in vivo processes. Rabbit oocytes from follicles, ovarian surfaces, and oviducts were fertilized...
In order to fertilize the egg, spermatozoa must go through the capacitation process where they experience Ca2+ uptake, increases in cyclic 3',5' adenosine monophosphate (cAMP) concentrations, supe...
Recent evidence indicated that human sperm capacitation is associated with an increased production of superoxide anion (O2.-). To further study the role and importance of O2.- in capacitation, we in...
The influence of nitric oxide on human sperm hyperactivation and capacitation, as well as its mechanism of action and its possible origin from spermatozoa were studied. Percoll-washed spermatozoa ...
The ability of bovine follicular fluid (FF) and oviduct fluid (ODF) to capacitate sperm was determined. At concentrations of greater than 20%, both FF and ODF capacitated sperm within 4 hours, but a...
A potentially important event during sperm capacitation is the loss of sperm membrane cholesterol. Although the exact mechanisms mediating this loss are not known, albumin and high density lipopr...
Semenogelin (Sg), the major protein of the human semen coagulum, is present at high concentrations in seminal vesicle secretions. It is degraded by the prostate-specific antigen (PSA) to generate pe...
Raf kinase inhibitor protein-1 (RKIP-1) belongs to the phosphatidyl ethanolamine–binding family of proteins (PEBP), which are highly conserved throughout evolution and widely expressed in tissues...
The signaling pathways that characterize the process of capacitation of human spermatozoa are still largely unknown. Modifications in the lipid architecture of the sperm plasma membrane have been de...
Sperm capacitation is regulated by multiple pathways that also control sperm motility and tyrosine (Tyr) phosphorylation of several sperm proteins. Among the reported pathways, phosphoinositide 3-ki...
Phosphodiesterases (PDEs) are enzymes that degrade cyclic nucleotides. The calcium-calmodulin dependent PDE type 1 (PDE 1) and the cyclic adenosine monophosphate (cAMP)-specific PDE type 4 (PDE 4) h...
Spermatozoa undergo a variety of changes during their life that are prerequisites to their maturation and ability to fertilize eggs. Mammalian sperm capacitation and acrosome reaction are regulated by...

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