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Isolation of highly purified type A spermatogonia from prepubertal rat testis
Type A spermatogonia cell separation c-kit cell culture
2009/2/17
We have developed a new method that allows isolation of highly purified type A spermatogonia from prepubertal rats. The procedure is based on the maximal release of spermatogonia from the seminifero...
Immunomagnetic isolation and long-term culture of mouse type A spermatogonia
Germ cells c-kit Ep-CAM magnetic beads Sertoli
2008/12/29
In the mammalian testis, type A spermatogonia proliferate and differentiate into sperm under the tight control of both endocrine and paracrine factors. In order to study the complex process of sperm...
Development of a Cryopreservation Protocol for Type A Spermatogonia
Freezing spermatogonial stem cells transplantation
2008/12/5
The aim of this study was to develop a cryopreservation protocol for type A spermatogonia. Testes from 5- to 7-month-old calves were collected, and type A spermatogonia were isolated using two-step ...