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Cryopreservation-Thawing of fractionated human spermatozoa is associated with membrane phosphatidylserine externalization and not DNA fragmentation
Phosphatidylserine translocation TUNEL annexin V
2008/12/29
The objective of these studies was to evaluate the effect of cryopreservation-thawing of human spermatozoa on DNA fragmentation and membrane integrity. This was a prospective, controlled cohort stud...
Comparison of Chromatin Assays for DNA Fragmentation Evaluation in Human Sperm
SCSA TUNEL SCD AOT
2008/12/23
Sperm chromatin integrity is vital for successful pregnancy and transmission of genetic material to the offspring. We evaluated chromatin integrity in sperm from 60 infertile men and 7 fertile don...
Reduction of the Incidence of Sperm DNA Fragmentation by Oral Antioxidant Treatment
Sperm DNA damage ejaculated spermatozoa TUNEL assay in vivo treatment vitamin C
2008/12/23
Sperm DNA fragmentation is known to compromise male fertility. Previous findings have suggested the implication of oxidative stress in the etiology of this pathological condition. The present study wa...
Reactive Oxygen Species and Cryopreservation Promote DNA Fragmentation in Equine Spermatozoa
DNA damage oxidative stress antioxidants horse sperm
2008/12/19
The objective of this study was to examine the effect of reactive oxygen species (ROS) and cryopreservation on DNA fragmentation of equine spermatozoa. In experiment 1, equine spermatozoa were incub...
Spontaneous DNA Fragmentation in Swim-Up Selected Human Spermatozoa During Long Term Incubation
DNA damage necrosis phosphatidylserine translocation reactive oxygen species sperm
2008/12/19
The origin and the meaning of DNA fragmentation in ejaculated human spermatozoa are not yet clear, although some hypotheses have been proposed. In the present study, we used investigated sperm DNA fra...
The Sperm Chromatin Dispersion Test: A Simple Method for the Determination of Sperm DNA Fragmentation
Structure human sperm decondensation
2008/12/18
Sperm DNA fragmentation is being increasingly recognized as an important cause of infertility. We herein describe the Sperm Chromatin Dispersion (SCD) test, a novel assay for sperm DNA fragmentation...