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HPV感染对男性精子活力及精子DNA完整性的影响
人乳头瘤病毒 精子活力 精子DNA完整性
2014/4/2
通过检测人乳头瘤病毒(HPV)感染的精液中精子活力及精子DNA完整性，探讨HPV感染对精液参数及精子DNA完整性的影响，进一步完善精液质量评估，为男性不育症患者，尤其是弱精子症者的治疗及辅助生殖技术助孕前的检查提供新的依据。方法 采用PCR-膜杂交法及染色质扩散实验法检测330例因不育就诊的男性患者精液中HPV感染率、感染亚型、精子活力及精子DNA完整性。根据精液中HPV的感染情况分为HPV感染组...
冷冻对人精子氧化应激水平、核DNA损伤的影响
精子冷冻 活性氧 氧化应激 DNA损伤
2012/6/29
研究冷冻储存对精子形态、氧化应激水平和核DNA损伤的影响。方法 根据WHO的标准（1999年），随机选取正常精液样本、异常精液样本各30例。分别检测精子冷冻前后正常形态率、丙二醛（MDA）浓度和DNA损伤率。结果 两组精液精子冷冻前后变化：冷冻后正常精液组中精子正常形态率降低明显，MDA值升高显著（P＜0.05）；DNA损伤率升高，但差异无统计学意义。而冷冻后异常精液组中精子正常形态率明显降低...
Flow cytometric DNA analysis of defined stages of rat seminiferous epithelial cycle during in vitro differentiation
Flow cytometric DNA analysis rat seminiferous epithelial cycle
2009/2/23
The stages of the rat seminiferous epithelial cycle have been isolated for flow cytometric analysis of DNA and for culture, using transillumination-assisted microdissection. Precise stages have been...
Evaluation of oxidative DNA damage in human sperm and its association with male infertility
8-Hydroxydeoxyguanosine spermatozoa infertility
2009/2/18
Recently, there is increasing evidence suggesting that oxidative sperm DNA damage is closely associated with impaired sperm function and male infertility. 8-Hydroxydeoxyguanosine (8-OHdG) is consi...
Rat seminal-vesicle secretory protein SVS II binds DNA with a preference for the 5' regulatory region of secretory protein SVS IV gene：co-isolation with components of the nuclear matrix
caltrin nuclear scaffold nuclear structure semen tissue
2009/2/18
In rats, the ventral prostate and seminal vesicles produce distinct sets of proteins whose functions and tissue-specific regulation by androgens remain unclear. We have utilized the genes encoding t...
Enhanced susceptibility of follicle-stimulating-hormone-deprived infertile bonnet monkey（Macaca radiata）spermatozoa to dithiothreitol-induced DNA decondensation in situ
Enhanced susceptibility follicle-stimulating-hormone-deprived infertile bonnet monkey dithiothreitol-induced DNA decondensation
2009/2/17
Immunoneutralization of endogenous follicle-stimulating hormone (FSH) of adult male monkeys leads to oligospermia and infertility despite unchanged testosterone levels. The inability of these monkey...
Effect of semen dilution on bovine sperm viability as determined by dual-DNA staining and flow cytometry
Sperm concentration cellular viability SYBR-14
2009/2/17
Living and dead spermatozoa were examined for the effects of sperm concentration level on sperm viability. Semen was collected from two different bulls on each of four collection dates. A ninth bull...
Mammalian sperm DNA susceptibility to in situ denaturation associated with the presence of DNA strand breaks as measured by the terminal deoxynucleotidyl transferase assay
Acrldine orange chromatin structure SCSA TdTA
2009/2/13
Sperm from four mammalian species were analyzed by the sperm chromatin structure assay (SCSA) and the terminal deoxynucleotidyl transferase assay (TdTA) using flow cytometry. The SCSA quantitates the ...
Dual DNA staining assessment of bovine sperm viability using SYBR-14 and propidium iodide
Sperm fluorescence SYBR-14 flow cytometry vital staining cryopreservation
2009/2/13
A new membrane-permeant DNA stain, SYBR-14, was used in combination with propidium iodide (PI) to estimate the proportion of living sperm in bovine semen. The SYBR-14 stained living sperm while PI...
Previous studies from this laboratory on hamster spermatozoa have demonstrated that rodent sperm DNA is packaged into the sperm nucleus in a specific manner by nuclear structures. The entire genom...
4-MAPC, a 5 alpha-reductase inhibitor, reduces rat ventral prostate weight, DNA, and prostatein concentrations
4-MAPC, a 5 alpha-reductase inhibitor rat ventral prostate weight DNA prostatein concentrations
2009/2/7
Several compounds, such as 4-MAPC (4-methyl-3-oxo-4-aza-5 alpha-pregnane-20- carboxylate), that inhibit conversion of testosterone (T) to dihydrotestosterone (DHT) by 5 alpha-reductase have been demon...
DNA-flow cytometry of defined stages of rat seminiferous epithelium: effects of 3 Gy of high-energy X-irradiation
testis Sertoli germ cell
2009/2/6
Testes of adult Sprague-Dawley rats were irradiated locally by 3 Gy of 4 MeV X-rays produced by a linear accelerator. This type and dose of radiation gives an even distribution through the testis an...
Cryopreservation-Thawing of fractionated human spermatozoa is associated with membrane phosphatidylserine externalization and not DNA fragmentation
Phosphatidylserine translocation TUNEL annexin V
2008/12/29
The objective of these studies was to evaluate the effect of cryopreservation-thawing of human spermatozoa on DNA fragmentation and membrane integrity. This was a prospective, controlled cohort stud...
Functional and ultrastructural features of DNA-fragmented human sperm
DNA-fragmented human sperm
2008/12/25
The functional significance of deoxyribonucleic acid (DNA) fragmentation in ejaculated human sperm is unclear. In this study the extent of DNA strand breakage in swim-up selected spermatozoa was eva...
An immunochemical assay to detect DNA damage in bovine sperm
immunochemical assay DNA damage bovine sperm
2008/12/25
An immunochemical assay has been developed to detect oxidative damage in bovine sperm DNA. Sperm DNA contains a large amount of oxidative damage as a result of exposure to exogenous agents, but dama...