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为评估定量荧光PCR (QF-PCR)方法在男性不育遗传学诊断中的应用价值, 文章对78例非梗阻性男性不育患者, 采用精液常规检测精子情况, 并检测患者性激素水平; 采用QF-PCR方法对患者性染色体多态性STR位点及特异性位点进行检测; 采用常规染色体G显带方法进行核型分析; PCR检测AZF微缺失。结果显示78例非梗阻性男性不育患者中发现无精子症患者18例, 少精子症患者20例, 总检出率为4...
The efficacy of quantitative fluorescent-polymerase chain reaction (QF-PCR) in the diagnosis of prenatal aneuploidy
Aneuploidy QF-PCR conventional karyotype analysis
2010/1/11
Aim: To investigate the efficacy of quantitative fluorescent-polymerase chain reaction (QF-PCR) in the diagnosis of aneuploidy. Materials and methods: The study included 40 pregnant women considered t...