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A simple, clinical assay to evaluate the acrosin activity of human spermatozoa
acrosin activity human spermatozoa
2009/2/23
Acrosin, a sperm-specific acrosomal proteinase, has an essential role in the fertilization process. Low levels of acrosin appear to be associated with subfertility and infertility, and the acrosin a...
Relationship of human sperm acrosin and proacrosin to semen parameters. II. Correlations
human sperm acrosin semen parameters
2009/2/23
Previous results, simultaneously confirmed by others, suggest that a relationship exists between sperm acrosin levels and fertility in man. The assessment of sperm acrosin may therefore be a useful ...
Comparison of glycerol and a zwitter ion buffer system as cryoprotective media for human spermatozoa. Effect on motility, penetration of zona-free hamster oocytes, and acrosin/proacrosin
spermatozoa human cryopreservation
2009/2/20
This study compared the cryoprotective effect of glycerol with that of a zwitter ion buffer system (TESTCY). Spermatozoa that are cryopreserved in the presence of glycerol possess a somewhat highe...
Immunocytochemical localization of acrosin on both acrosomal membranes and in the acrosomal matrix of porcine spermatozoa
spermatozoa acrosin electron microscopy
2009/2/19
Immunocytochemical techniques were employed to determine, at the ultrastructural level, the location of acrosin in porcine spermatozoa. Antisera to highly purified porcine acrosin was produced in ...
Relationship of Human Sperm Acrosin and Proacrosin to Semen Parameters. I. Comparisons Between Symptomatic Men of Infertile Couples and Asymptomatic Men, and Between Different Split Ejaculate Fractions
acrosin proacrosin semen spermatozoa split ejaculate fertility infertility
2009/2/19
Acrosin is a sperm serine proteinase whose activity appears to be essential for fertilization in mammals, although this has not been shown in man due to experimental limitations. The present stu...
Glycosaminoglycan Stimulation of the In Vitro Conversion of Boar Proacrosin into Acrosin
acrosomal enzymes acrosome reaction proacrosin acrosin glycosaminoglycan
2009/2/18
Glycosaminoglycans obtained from shark cartilage, whale cartilage, porcine intestinal mucosa, porcine skin, and human umbilical cord were found to accelerate the in vitro conversion of highly puri...
Acrosin, Proacrosin, and Acrosin Inhibitor of Human Spermatozoa：Extraction, Quantitation, and Stability
acrosin proacrosin acrosin inhibitor human spermatozoa
2009/2/18
Ten different methods were evaluated to determine the treatment for obtaining maximal amounts of acrosin, proacrosin, and acrosin inhibitor (the acrosin system) from human spermatozoa by a single te...
Evidence of proacrosin molecule abnormality as a possible cause of low acrosin activity and unexplained failure of fertilization in vitro
Spermatozoa male infertility immunoblotting
2009/2/17
The aim of this study was to investigate whether the molecular abnormality of proacrosin is associated with an unexplained failure of in vitro fertilization (IVF) and low acrosin activity in human...
Effects of substrate and separation method on acrosin amidase measurements
Enzyme activity DL-BAPNA L-BAPNA swim-up
2009/2/16
The purpose of this study was to increase the accuracy and reproducibility of the acrosin amidase assay and to assess the effects of different methods of sperm isolation on total sperm acrosin act...
Acrosin activity of human spermatozoa by means of a simple gelatinolytic technique：a method useful for IVF
Human spermatozoa acrosin activity male infertility
2009/2/13
Acrosin activity was determined using a gelatinolysis technique in 100-microliter semen aliquots of 114 patients (normozoospermia, n = 90; asthenozoospermia, n = 12; oligozoospermia, n = 10; polyz...
Human sperm acrosin. Further studies with the clinical assay and activity in a group of presumably fertile men
spermatozoa acrosin Triton X-100 fertility
2009/2/11
The goals of this study were to determine the effect of the nonionic detergent, Triton X-100, on the recovery of acrosin in the clinical assay (Kennedy et al, 1989), since previous investigations ...
Biochemical alterations in the proacrosin-acrosin system during epididymal maturation of the rat spermatozoa
Biochemical alterations proacrosin-acrosin system epididymal maturation rat spermatozoa
2009/2/10
Acrosin, an acrosomal serine protease, is believed to have a role in fertilization. The enzyme is synthesized in an enzymatically inactive precursor form, proacrosin, and is processed to enzymatical...
In vitro interactions of calmodulin with the ovine proacrosin-acrosin system
acrosome spermatozoa proteolytic activity
2009/1/5
The authors studied the interaction of calmodulin (CaM) with proacrosin and acrosin from ram spermatozoa. CaM binding evaluated by the [125I]-CaM overlay procedure was shown to occur preferentially ...