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New microscope technique reveals internal structure of live embryos（图）
New microscope technique internal structure live embryos
2017/9/4
University of Illinois researchers have developed a way to produce 3-D images of live embryos in cattle that could help determine embryo viability before in vitro fertilization in humans.Infertility c...
Green Light-emitting Diodes Light Stimuli during Incubation Enhances Posthatch Growth without Disrupting Normal Eye Development of Broiler Embryos and Hatchlings
Chick Embryo Incubation Light Emitting Diodes Light Stimuli
2016/11/10
Monochromatic green light-emitting diodes (LED) light stimuli influences the posthatch growth performance of chicks. This study was undertaken with the following objectives: i) to examine whether the ...
Hypodiploidy as a prominent attributor to chromosomal aneuploidy in transgenic rabbit embryos
chromosomal aneuploidy EGFP embryo hFVIII rabbit transgenic
2015/6/5
Transgenic animals play a vital role in basic research, agriculture and pharmaceutical industries. Rabbits have the advantage of other large laboratory species in that they have a short gestation peri...
Single fixed-time laparoscopic intrauterine insemination as a tool to obtain low-diversity porcine embryos
laparoscopic insemination embryo diversity pig
2015/4/16
Double fixed-time insemination after ovulation induction is commonly used in pigs to obtain in vivo produced embryos at defined stages of development for downstream biotechnological applications. Howe...
Conception rate after sex determination and cryopreservation of D7 bovine embryos
cattle superovulation sexing
2015/4/8
The aim of this study was to evaluate conception rate after sex determination and subsequent freezing of Day 7 (D7) bovine embryos and to compare it with transfer of fresh female embryos. High quality...
Lower quality bovine embryos may be successfully used for sex determination
cattle embryo superovulation sex determination polymerase chain reaction embryo transfer
2015/3/13
The aim of this study was to evaluate the effect of sex determination procedures in Day 7 to Day 8 bovine embryos of various quality. For the purposes of comparison we used high quality (HQ) as well a...
The effect of vitrification in open pulled straws on pregnancy rates after transfer of in vivoproduced bovine embryos
bovine embryo superovulation cryopreservation freezing thawing vitrification embryo transfer
2015/3/5
The aim of this study was to compare pregnancy rates after transfer of in vivo produced embryos cryopreserved using open pulled straw (OPS) vitrification (Group V) or conventional freezing method as a...
Collection of oocytes from donors in the growth phase of follicular development can enhance the production of bovine embryos for cryopreservation
follicular development bovine oocyte in vitro embryos cryopreservation
2015/3/5
The present study was designed to compare the efficiency of bovine embryo production for cryopreservation between oocytes collected from donors in the growth phase of follicular development (GPFD) and...
Altered pathogenicity, immunogenicity, tissue tropism and 3'-7 kb region sequence of an avian infectious bronchitis coronavirus strain after serial passage in embryos
vian infectious bronchitis coronavirus Pathogenicity Immunogenicity Tissue tropism Genome sequence Serial passage
2016/12/20
In this study, we attenuated a Chinese LX4-type nephropathogenic infectious bronchitis virus (IBV) strain, CK/CH/LHLJ/04V, by serial passage in embryonated chicken eggs. Based on sequence analysis of ...
Effect of Estradiol and Nonylphenol on mRNA Levels of VitellogeninII in the Liver of Chicken Embryos
nonylphenol estradiol vitellogenin II chicken embryo RT-PCR
2009/8/3
Nonylphenol is one of the endocrine disrupting agents with estrogenic activity in some vertebrates. The present study was conducted to assess estrogenic activity of p-nonylphenol (NP) in chicken embry...
Population of Circulating Primordial Germ Cells in Early Japanese Quail Embryos
primordial germ cells circulation erythrocytes quail embryo
2009/8/3
Avian primordial germ cells (PGCs) show a unique migration pathway during early development. As soon as the blood vessels have formed, PGCs enter the circulatory system, and migrate to the gonadal pri...
Fate of the Donor Blastodermal Cells Derived from the Central Disc,Marginal Zone and Area Opaca Transferred into the Recipient Embryos
chicken blastodermal cell GFP gene gene expression lipofection
2009/8/3
In order to trace the donor blastodermal cell movement in the recipient embryos, stage X blastodermal cells derived from the central disc (CD), marginal zone (MZ) and area opaca (AO) were transfected...
Effect of Estradiol and Nonylphenol on mRNA Expression of Estrogen Receptors α and β,and Cytochrome P450 Aromatase in the Gonad of Chicken Embryos
p-nonylphenol ERα ERβ P450arom endocrine disruptor
2009/7/31
Effects of estradiol-17β (E2) and p-nonylphenol (NP) on mRNA expression of estrogen receptor α (ERα), estrogen receptor β (ERβ) and cytochrome P450 aromatase (P450arom) were examined in the early stag...
Efficient Gene Transfer into Early Chicken Embryos by Electroporation of Stage X Blastoderms in Vivo, Applying Electric Pulses Vertically to the Blastoderm Layer
blastoderm chick embryo electroporation embryo culture GFP gene
2009/7/31
In order to introduce exogenous DNA into early chicken embryos by transfecting the stage X (EG&K) blastoderm, in vivo electroporation was applied to it. A pair of novel electrodes was devised for appl...
A Simple Method for Sexing Chicken Embryos at Stage X
blastodermal cell chicken embryo embryo culture PCR sexing
2009/7/31
A simple method for sexing chicken embryos was devised using DNA prepared from blastodermal cells. A cluster of cells was collected from the area opaca of a stage X blastoderm, and DNA was prepared by...