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‘阳光玫瑰’葡萄病毒小RNA测序鉴定及RT-PCR检测
阳光玫瑰 小RNA测序 葡萄病毒 RT-PCR
2019/12/25
‘阳光玫瑰’是我国从日本引进的葡萄优良品种。为了明确我国‘阳光玫瑰’葡萄病毒病的病原,本研究采用小RNA测序技术对2株显症和无症状的‘阳光玫瑰’葡萄样品进行病毒鉴定结果显示:显症样品中测定到8种病毒,其中包含葡萄蚕豆萎蔫病毒(Grapevine fabavirus, GFabV)和灰比诺葡萄病毒(Grapevine Pinot gris virus, GPGV);无症状样品中测定到3种葡萄病毒。对...
应用实时荧光定量RT-PCR高效检测葡萄病毒B
葡萄 葡萄病毒B 实时荧光定量RT-PCR 常规RT-PCR
2019/11/18
本研究建立了葡萄病毒B的 SYBR GreenⅠ实时荧光定量RT-PCR(RT-qPCR)检测技术。该技术标准曲线扩增效率102.4%,相关系数0.999,最低检测限达10-4倍稀释cDNA,灵敏度为常规RT-PCR的100倍。重复性试验组内和组间变异系数分别为0.00%~0.65%和0.02%~2.00%,表明检测稳定性好。该技术对田间葡萄样品检测适用范围广,对枝条和老叶柄检测效果最好,冬季枝条...
3种甘薯病毒多重RT-PCR检测方法的建立及应用
Sweet potato virus multiplex RT-PCR Sweet potato feathery mottle virus Sweet potato latent virus Sweet potato virus 2
2020/2/12
To effectively monitor the occurrence of major sweet potato viruses, the multiplex RT-PCR assay was established for detection of Sweet potato feathery mottle virus (SPFMV), Sweet potato latent virus (...
Simultaneous detection of three Potyvirus species from Cucurbitaceae crops by multiplex RT-PCR
Watermelon mosaic virus (WMV) Zucchini yellow mosaic virus (ZYMV) Papaya ringspot virus (PRSV) Multiplex RT-PCR
2009/3/17
A multiplex RT-PCR procedure was developed for the simultaneous detection of Watermelon mosaic virus (WMV), Zucchini yellow mosaic virus (ZYMV) and Papaya ringspot virus (PRSV) from Cucurbitaceae cr...