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Sequence analysis of the mtDNA region correlated with Shaan 2A cytoplasmic male sterility in rapeseed (Brassica napus L.)
cytoplasmic male sterility (CMS) marker-assisted selection (MAS) orf224-1 rapeseed (Brassica napus L.) Rf-restorer gene Shaan 2A CMS line
2014/2/25
Until now in Europe has not been cultivated any hybrid cultivar of oilseed rape based on the Shaan 2A cytoplasmic male sterility (CMS), a widely used CMS system in China. The aim of Czech breeders now...
Origin of Local Radish Variety,‘Sabaka’,in Kyoto Prefecture Inferred by Analyses of Genes for Cytoplasmic Male-sterility and Fertility Restoration
domestication Japanese wild radish mitochondrial orf138 orf687 PCR-RFLP
2009/7/6
To clarify the origin of local radish variety ‘Sabaka’ in Kyoto prefecture, the presence and variations of mitochondrial male-sterile gene (orf138) and the fertility restorer gene, orf687, were invest...
花椰菜细胞质雄性不育基因特异PCR标记的筛选Identification of PCR markers associtated with cytoplasmic male sterility in Brassica oleracea var botrytis
花椰菜(Brassica oleracea var.Botrytis) 细胞质雄性不育 CMS 候选基因
2008/1/10
摘要
基于同源序列的候选基因法(homology-based candidate gene method),通过检索NCBI核酸及蛋白数据库,获得细胞质雄性不育(cytoplasmic male sterility CMS)相关的基因或开放读码框。生物学软件分析,根据保守区设计5对特异引物,PCR扩增,其中引物P9/P10在花椰菜细胞质雄性不育系knxd612中特异扩增出313 bp的片段。单株...