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Studies on Culture of Cells and Tissues of Crop Plants:III. Isolation of rice mesophyll protoplasts with a new cell wall lytic enzyme "funcelase"
Cell wall Cellulase "Onozuka" R-10 Funcelase Mesophyll Oryza sativa L
2009/8/6
Rice mesophyll protoplasts were isolated with ease and with high yield from expanded 2nd leaves, which were grown on agar plates under 23°C, 16.5 W/m2 for 25 days, by using a new cell wall lytic enzym...
A Cyto-histological Study on Shoot Differentiation and Mesophyll Morphology of the Excised Lettuce (Lactuca sativa L.) Cotyledons as Affected by Light Conditions during Seedling Growth
Cell Cotyledon Lettuce Light Mesophyll Shoot differentiation
2009/8/6
Light quality and quantity of the environment, where lettuce seedlings were grown, affected the mesophyll tissue and the shoot formation in cotyledons excised and cultured in vitro. When cultured on M...
An Efficient Method for Callus Formation of Protoplasts from Peanut (Arachis hypogaea L.) Leaf Mesophyll
Callus formation Mesophyll protoplast Peanut Protoplast culture
2009/7/30
High yields of viable protoplasts were obtained from leaf mesophyll of 10-day-old peanut seedlings (Arachis hypogaea L. var. Lokal). An enzyme solution containing 1% Cellulase YC, 2% Meicelase P-1, 0....
Ultrastructural Changes of Chloroplasts in Peanut Mesophyll Protoplasts Treated with Electric Fields
Chloroplast division Chloroplast fusion Electric fields Mesophyll protoplasts Peanut
2009/7/28
Ultrastructural changes in chloroplasts of peanut (Arachis hypogaea L. var. Lokal) seedling mesophyll cells and mesophyll protoplasts treated with electric fields were investigated. Generally, chloro...
Differential Sensitivity of Chloroplasts in Mesophyll and Bundle Sheath Cells in Maize,an NADP-Malic Enzyme-Type C4 Plant,to Salinity Stress
Bundle Sheath C4 Plant Chloroplast Maize Mesophyll Salinity
2009/7/27
The changes in chloroplast ultrastructure and the contents of chlorophyll, Na and K in response to salinity stress were investigated in leaves of maize, an NADP-malic enzyme-type C4 plant species poss...
Gene Expression and Accumulation of Rubisco in Bundle Sheath and Mesophyll Cells during Leaf Development and Senescence in Rice,a C3 Plant
Bundle sheath C3 photosynthesis Chlorophyll fluorescence Immuno-localization In situ hybridization Leaf development Leaf senescence Mesophyll
2009/7/23
Gene expression of ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) large subunit (rbcL) and small subunit (rbcS) in bundle sheath and mesophyll cells of rice, a C3 plant, was examined during...
A Simple Procedure for Mesophyll Protoplast Culture and Plant Regeneration in Brassica oleracea L. and Brassica napus L
Brassica napus L Brassica oleracea L organogenesis protoplast culture regeneration
2014/3/13
An improved protocol for Brassica protoplast culture and plant regeneration was developed. Isolated
protoplasts from four-weeks-old in vitro shoot tip culture of Brassica oleracea var. botrytis cv...