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The pathogenic bacterial genus Xanthomonas infects a wide variety of host plants and causes devastating diseases in many crops. Transcription activator-like effectors (TALEs) are important virule...
In North America, Gremmeniella abietina, European race (GaEU), was reported in 1975. Our objective was to follow the spread of GaEU on red pines growing on flat land and on slope.
In North America, Gremmeniella abietina, European race (GaEU), was reported in 1975. Our objective was to follow the spread of GaEU on red pines growing on flat land and on slope. Annual height infect...
Tomato is one of the most important vegetable crops in Nepal. Duringthe spring of 2009, dark, circular and water-soaked lesions of about 3 mm in diameter were observed on leaves of tomato plants (Sola...
Many species of soil-inhabiting fungus Fusarium, cause severe yield loss in many crops. Experiments were conducted in net house condition with complete randomized block design to determine the individ...
To evaluation of pathogenicity and race classification of Xanthomonas oryzae pv. oryzae agent bacterial leaf blight of rice, 153 isolates of X. oryzae pv. oryzae were collected from different rice-gro...
Four economically important genera in the Cucurbitaceae family, namely Citrullus, Cucumis, Cucurbita and Langenaria, have no resistance to Meloidogyne species, which cause extensive crop losses. Certa...
Cotton, which is also known as ‘white gold’, is an important crop in many developing countries. The crop yield is dependent on the environment in which it is grown. One of the major challenges for cul...
The plant-pathogenic prokaryote Xanthomonas oryzae pv. oryzae (Xoo) causes bacterial blight, one of the most important diseases of rice. The bacterium is a model organism for the analysis of plant-pat...
A rootstock cultivar of melon (Cucumis melo L.) ‘Dodai No. 1’, which shows remarkable quantitative resistance to race 1,2y of Fusarium oxysporum f.sp. melonis. was developed from a cross between ‘Melo...
Two new rootstock cultivars of melon (Cucumis melo L.) resistant to race 1,2y of Fusarium oxysporum f.sp. melonis were released. ‘Dodai No. 1’ was selected from a cross between ‘Melon Parental Line 1’...
[ 目的] 运用分子生物学技术克隆猪GPX2 基因。[ 方法] 以猪十二指肠总RNA 为模板, 根据人、大鼠、小鼠、牛、狗GPX2 基因同源 序列分析, 设计兼并引物对, 采用RT- PCR 技术扩增获得了1 段330bp 的猪GPX2 基因序列。根据获得的已知序列分别设计引物, 采用 3′- RACE 和5′- RACE 技术手段分离、克隆猪GPX2 基因。并对所得基因进行基因序列分析。[ ...
[ 目的] 为研究Dmrt 基因的进化、尼罗罗非鱼性别决定机制和功能提供资料。[ 方法] 以尼罗罗非鱼精巢cDNA 为模板, 采用简并 PCR 扩增和克隆其Dmrt 保守区域, 并采用3′RACE 反应获得Dmrt1 基因全序列。[ 结果] 尼罗罗非鱼中Dmrt 保守区域长度为158 bp 尼 罗罗非鱼Dmrt1 基因的DM-domain 与奥利亚罗非鱼、虹鳟、青鱼将、新月鱼、人、小鼠中Dmr...
综述了RACE 的技术原理、局限性、方法改良和新型的RACE 技术, 并且讨论了RACE 技术在植物基因研究中的技术优势、应用现 状和发展前景。
Fusarium oxysporum f. sp. vasinfectum (Atk.) W.C. Snyd. & H. N. Hans. race 4, a biotype highly virulent on certain Pima cotton (Gossypium barbadense L.) cultivars, was detected in California in 2001. ...

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