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Restoration of Genetic Resources from Ehime Native Chicken via Transferred Primordial Germ Cells (PGCs)
chimeras germ line primordial germ cell embryos
2009/8/3
This experiment was designed to produce chimeric chicken by transferring primordial germ cells (PGCs) of Ehime native chicken embryos to White Leghorn ones. Fertilized eggs produced by both chicken br...
Conservation of a Genetically Selected Broiler Line (42L)Using Cryopreserved Circulating Primordial Germ Cells (PGCS) Isolated by Filtration Method
Filtration method PGCs cryopreservation germ-line chimeras chicken
2009/7/31
A genetically selected broiler line (42L) developed at the Pennsylvania State University, PA, USA was used in the present study. In Experiment 1, embryonic blood was collected from chick embryos at ...
Effects of Ultraviolet Irradiation on the Migratory Ability of Primordial Germ Cells (PGCs) in the Domestic Chicken
UV irradiation PGCs Migratory ability embryo chicken
2009/7/31
The effect of ultraviolet (UV) irradiation on the ability of primordial germ cells (PGCs) to migrate toward the gonadal ridge was investigated in the domestic chicken.
When PGCs were irradiated with ...
Effect of Transfer Primordial Germ Cells (PGCs) into Chick Gonad
chicken embryo gonad PGCs transfer
2009/7/30
Primordial germ cells (PGCs) of white leghorn Maria line hens embryos as a donor were transferred white leghorn Laura line hens embryos as a recipient. During stage 12 to 15 of incubating fertilized ...
Cryopreservation of Primordial Germ Cells (PGCs) from White Leghorn Embryos Using Commercial Cryoprotectants
chicken cryoprotectants PGCs recovery-rate viability
2009/7/30
Cryopreservation of poultry semen has been reported, but preservation of female genetic material has not been possible because of the large size of their yolk structure. Cryopreservation of primordial...
Depletion of Primordial Germ Cells (PGCs) by X-irradiation to Extraembryonic Region of Chicken Embryos and Expression of Xenotransplanted Quail PGCs
chicken chimera primordial germ cell quail soft x-ray
2009/7/29
The generation of germline chimeras by the transfer of primordial germ cells (PGCs) requires incorporation of the PGCs of the donor into the gonadal tissue of the recipient embryo. We investigated the...
鸡胚原始生殖细胞（PGCs）玻璃化冷冻的研究
玻璃化冷冻 原始生殖细胞 鸡胚
2008/4/22
对发育至第19期和第28期鸡胚性腺原始生殖细胞（primordial germ cells ，PGCs），用六种玻璃化冷冻液I：10%DMSO+10%EG+10%PVP，II：20%EG+10%PVP，III：20%DMSO+10%PVP，IV：10%DMSO+10%EG+0.5mol/LSurcose，V：20%EG+0.5mol/LSurcose，VI：20%DMSO+0.5mol/LSurc...