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Species difference in the expression of Fas and Fas ligand in mature mammalian spermatozoa
sperm Fas antigen FasL
2017/12/25
Although it has been proposed that the Fas and Fas ligand (FasL) may protect ejaculated spermatozoa against apoptosis induced by lipoperoxidative damage and against lymphocytes present in the female g...
Effect of low-density lipoprotein addition to soybean lecithin-based extenders on bull spermatozoa following freezing-thawing–preliminary results
bull sperm cryopreservation sperm motility viability cryoprotectant
2016/12/20
Soybean lecithin-based extenders represent an alternative to extenders containing egg yolk, but there has been discussion about their cryoprotective efficacy. Low-density lipoprotein (LDL) was proved ...
Lead induced alterations in rabbit spermatozoa motility and morphology in vitro
reproductive toxicity rabbit Pb temperature sperm motility morphological abnormality
2016/11/11
The aim of this in vitro study was to determine the effect of lead chloride (PbCl2) on rabbit spermatozoa motility and morphology. Lead concentrations in the medium ranged between 0.45 and 11.17 μg/ml...
Cryopreservation of fluorescence activated cell sorted boar spermatozoa based on extracellular ubiquitination
pig antibody staining quality marker porcine sperm FACS ubiquitin cryopreservation
2016/8/1
The present study is focused on the methodology of fluorescence activated cell sorting (FACS) of spermatozoa stained by the antibody against extracellular surface marker ubiquitin (eUb) and subsequent...
Motility of boar spermatozoa supplemented with homologous seminal plasma of high or low protein content after storage for three days
seminal plasma homologous autologous
2016/2/23
The effect of adding high or low protein content homologous seminal plasma to boar spermatozoa on the progressive motility of the spermatozoa after storage for three days, and at a 1 : 4 dilution rati...
Kinetics of hydrogen peroxide generated from live and dead ram spermatozoa and the effects of catalase and oxidase substrates addition
reactive oxygen species ram antioxidant
2016/1/29
The generation of hydrogen peroxide (H2O2) by ram spermatozoa (spz) was measured using a flurometric assay with 10-acetyl-3,7-dihydroxyphenoxazine agent as a probe for H2O2 detection. The kinetics of ...
Relationship between acrosome integrity changes and in vitro fertilising ability of bovine spermatozoa
bull spermatozoa acrosome embryos IVF
2015/10/9
This study was designed to investigate the characteristics of acrosomal changes during capacitation of bovine spermatozoa in relationship to in vitro fertility of individual bulls. Motile spermatozoa ...
Exploration of natural cryoprotectants for cryopreservation of African catfish, Clarias gariepinus, Burchell 1822 (Pisces: Clariidae) spermatozoa
egg yolk honey glucose coconut water
2015/7/7
Toxicity is a major limitation to successful spermatozoa cryopreservation of fish. Due to this
problem, it is critical to find potential cryoprotectants which are more environmental-friendly, non-to...
Oxidative stress and motility in tench Tinca tinca spermatozoa
tench sperm activating medium osmolality
2015/7/7
It was hypothesized that sperm hypotonic exposure can provoke oxidative
stress which could be involved in sperm quality degradation. Our study aimed to describe spermatozoa motility
parameters and...
Comparison of oxidant and antioxidant status of seminal plasma and spermatozoa of several fish species
antioxidant enzymes oxidative stress fish sperm
2015/6/24
Oxidant and antioxidant activity in seminal plasma was compared with that in spermatozoa of teleost (common carp Cyprinus carpio and brook trout Salvelinus fontinalis) and chondrostean (Russian sturge...
Relationship between abnormal spermatozoa and seminal plasma free amino acids in boars
boars semen morphologically abnormal spermatozoa seminal plasma free amino acids
2015/6/4
The objective of this study was to provide some data on concentrations of seminal plasma free amino acids (FAAs) in order to compare these values with different total contents of morphologically abnor...
Effect of long-term storage on induced photon emission of boar spermatozoa
boar semen oxidative stress luminescence photon emission sperm motility
2015/6/2
Ultraweak photon emission measurements were found to be a useful tool for investigating the influence of stress factors on cells. In the present study, induced photon emission of boar spermatozoa was ...
DNA integrity in fresh, chilled and frozen-thawed canine spermatozoa
dog DNA fragmentation index sperm chromatin structure assay (SCSA) cryopreservation chilled semen
2015/4/16
Sperm chromatin status in fresh dog semen and the effect of long-term storage of chilled and frozen dog semen on sperm chromatin integrity was assessed by the sperm chromatin structure assay (SCSA). I...
Effect of acrosome reaction progress in frozen-thawed boar spermatozoa on the efficiency of in vitro oocyte fertilization
pig cryopreserved sperm assessment
2015/4/7
A good functional status of cryopreserved boar spermatozoa is very important for successful fertilization of porcine oocytes and in vitro embryo production. The purpose of the study was to evaluate th...
Pig Spermatozoa Defect in Acrosome Formation Caused Poor Motion Parameters and Fertilization Failure through Artificial Insemination and In vitro Fertilization
Boar Sperm Motion Parameters In vitro Fertilization Acrosome Defect
2016/5/17
The selection of morphologically normal spermatozoa is critical to obtain high breeding performances in boar breeding farms and artificial insemination (AI) centers. Parameters for the selection of se...