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Inhibition of c-Jun N-terminal kinase (JNK) suppresses porcine oocyte ageing in vitro
MAPK DNA fragmentation apoptosis Bax
2015/6/24
Oocyte ageing is a complex of processes that occur when matured in vitro oocytes are, after reaching the metaphase II stage, exposed to further in vitro culture. Aged oocytes remaining at the metaphas...
Effects of oocyte collection techniques and maturation media on in vitro maturation and subsequent embryo development in Boer goat
Boer goat collection techniques oocyte maturation ovary medium
2015/6/4
The oocytes (experiment 1) were harvested by one of the four collection techniques (slicing, puncture, aspiration I and aspiration II) and the total number and the number of each grade of oocytes were...
CHARACTERIZATION OF Ca2+ INFLUX PATHWAY(S) DURING MOUSE OOCYTE MATURATION
Calcium influx oocyte maturation SOCE Stim1 Phosphorylation
2014/12/10
Ca2+ signaling induced at fertilization, also known as oscillations, is essential in mammalian eggs to initiate early embryonic development. The generation of the oscillations relies on optimization o...
Siberian Sturgeon Oocyte Extract Induces Epigenetic Modifications of Porcine Somatic Cells and Improves Developmental Competence of SCNT Embryos
Epigenetic Modifications Somatic Cell Nuclear Transfer Siberian Sturgeon Oocyte Extract
2016/5/16
Somatic cell nuclear transfer (SCNT) has generally demonstrated that a differentiated cell can convert into a undifferentiated or pluripotent state. In the SCNT experiment, nuclear reprogramming is in...
Effects of Trichostatin A on Cumulus Expansion during Mouse Oocyte Maturation
Mouse Cumulus-oocyte Complex Cumulus Expansion TSA
2016/5/12
This study was conducted to investigate the effects of Trichostatin A (TSA) on cumulus expansion during mouse oocyte maturation. TSA treatment inhibited cumulus expansion and significantly reduced the...
Ovarian Follicular Dynamics, Ovarian Follicular Growth, Oocyte Yield, In vitro Embryo Production and Repeated Oocyte Pick Up in Thai Native Heifers Undergoing Superstimulation
FSH Follicle OPU COCs IVP Cattle
2016/5/11
The objective of this study was to compare the effectiveness of the protocols for superstimulation of follicular growth in Thai native heifers. Heifers (n = 20) were randomly divided into four groups ...
Comparison of Gene Expression between Cumulus Oocyte Complexes and Naked Oocytes by Suppression Subtractive Hybridization in Swine
Suppression Subtractive Hybridization (SSH) Gene Expression Cumulus Cells Porcine
2016/5/3
In the antral follicle phase, several layers of cumulus cells surround the oocyte and play an important support and regulation role in oocyte development and maturation via intercellular communication...
Regulatory Mechanism of Spindle Movements during Oocyte Meiotic Division
Spindle Movement Meiotic Division Cytoskeleton Oocyte
2016/5/3
Female germ cell meiotic divisions are typically asymmetric, giving rise to two daughter cells with different sizes. Spindle movements including spindle migration from the oocyte center to the cortex ...
An Antisense Oligodeoxynucleotide to the LH Receptor Attenuates FSH-induced Oocyte Maturation in Mice
Cumulus-enclosed Oocyte LH Receptor Oligodeoxynucleotide Mouse
2016/4/28
It has been recently shown that expression of the LH receptor (LHR) in cumulus cells is related with FSH-induced meiotic resumption of mouse cumulus enclosed oocytes (CEOs). However, to date, it is st...
Effect of Oocyte Activation Regimens on Ploidy of Nuclear Transfer Embryos Reconstructed with Fetal Fibroblasts in Rabbit
Activation Nuclear Transfer Development Ploidy Rabbit
2016/4/25
Considerable attention has been focused on the cloning of mammalian embryos, as a consequence of poor development, in order to enhance the application of genetic engineering. Experiments were conducte...
Effect of Monosaccharide L-fucose and Polysaccharide Fucoidan on Sperm L-fucosidase Activity and Relation to Sperm-oocyte Interaction in Pig
Pig Oocytes Chondroitin Sulfate Sperm Penetration In vitro
2016/4/25
Carbohydrate-protein interactions are known to be important in gamete interactions. Several evidence indicated that a fucose-containing sulfated polysaccharide fucoidan was potential inhibitor of fert...
Vascular Endothelial Growth Factor Has Beneficial Effect Independent of Serum Components throughout Oocyte Maturation and Early Embryonic Development in Cattle
Bovine Embryo Early Development IVM-IVF-IVC Serum VEGF
2016/4/21
In our previous studies, we demonstrated that Vascular Endothelial Growth Factor (VEGF) enhances bovine oocyte maturation and early embryonic development in serum supplemented media. In this experimen...
Isolation of an Oocyte Stimulatory Peptide from the Ovarian Follicular Fluid of Water Buffalo (Bubalus bubalis)
Follicular Fluid Peptide Oocyte IVM Buffalo
2016/4/20
Ovarian follicular fluid contains both stimulatory and inhibitory agents that influence the growth and maturation of oocyte. In the present study, an attempt was made to isolate and study the biologic...
Surgical Oocyte Retrieval and the Developmental Potential of the Oocytes Derived from Prepubertal Calves
Oocyte Gonadotropin Calf Surgical Retrieval
2016/4/14
The objectives of this study were to investigate the ovarian responsiveness of juvenile calves to exogenous gonadotropin treatments and to establish the oocyte retrieval technique for prepubertal heif...