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Siberian Sturgeon Oocyte Extract Induces Epigenetic Modifications of Porcine Somatic Cells and Improves Developmental Competence of SCNT Embryos
Epigenetic Modifications Somatic Cell Nuclear Transfer Siberian Sturgeon Oocyte Extract
2016/5/16
Somatic cell nuclear transfer (SCNT) has generally demonstrated that a differentiated cell can convert into a undifferentiated or pluripotent state. In the SCNT experiment, nuclear reprogramming is in...
Optimization of Procedure for Efficient Gene Transfer into Porcine Somatic Cells with Lipofection
Lipofection Porcine Developmental Competence Somatic Cell Nuclear Transfer (SCNT)
2016/4/27
The objective of this study was to establish conditions for transfection of a foreign gene into somatic cells using cationic lipid reagents and to evaluate the effects of transfection on in vitro deve...
Production of Cloned Calves by the Transfer of Somatic Cells Derived from Frozen Tissues Using Simple Portable CO2 Incubator
Cloning Frozen Fetal Skin Electrofusion Portable CO2 Incubator Blastocysts Calves
2016/4/14
The ability of frozen-thawed fetal skin was examined to generate viable cell lines for nuclear transfer. Fetal skin frozen at -20째C, -30째C or -80째C in the presence of 5% DMSO used...
Comparison of Natural Resistance-Associated Macrophage Protein (NRAMP)1 Expression between Cows with High and Low Milk Somatic Cells Counts
NRAMP1 RT-PCR Bovine mastitis Staphylococcus aureus
2016/4/14
Studies using natural resistance-associated macrophage protein (NRAMP) identification indicated that cattle could be selected for immunity. Several studies performed on intracellular organisms such as...
