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Stark Effect Spectroscopy of the Rhodobacter sphaeroides Reaction Center Heterodimer Mutant
Rhodobacter sphaeroides Macromolecular Substances Light-Harvesting Protein Complexes Photosynthetic Reaction Center Complex Proteins Bacterial Proteins Spectrophotometry Mutation Thermodynamics
2016/5/23
The effect of an electric field has been measured on the absorption spectrum (Stark effect) of the heterodimer mutant (M)H202L of Rhodobacter sphaeroides reaction centers, where the primary electron d...
Electric Field Modulation of the Fluorescence Spectrum from Rhodobacter Sphaeroides Reaction Centers
fluorescence counterstain nuclear counterstain immunocytochemistry propidium iodide cell culture
2016/5/20
The fluorescence intensity from randomly oriented Rhodobacter ( Rb.) sphaeroides photosynthetic reaction centers at 77 K increases in an applied electric field. This is ascribed to a net reduction in ...
摘要 RhodobactersphaeroideshemA编码5氨基乙酰丙酸合酶(ALAS),催化磷酸吡哆醛依赖性琥珀酰CoA和甘氨酸缩合成ALA.将R.spaeroideshemA导入E.coli进行表达,当hemA具有与lac启动子相同的转录方向时,ALAS有活性.lac启动子与hemA之间的距离会影响ALAS在不同培养基上的表达.E.coli宿主菌对ALAS表达、ALA产量有显著影响,在实验...
