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Living Cell Membranes Can Self-Sort Their Components By ‘Demixing’（图）
Living Cell Membranes Self-Sort Components Demixing
2017/12/19
Cells — the building blocks of our bodies — are encapsulated by membranes. The same goes for the specialized compartments within our cells.These membranes are extremely thin, oily films, containing pr...
Formation and Analysis of Topographical Domains Between Lipid Membranes Tethered by DNA Hybrids of Different Lengths
Cell Membrane DNA Membrane Lipids Lipid Bilayers 1,2-Dipalmitoylphosphatidylcholine Microscopy, Fluorescence Base Sequence Molecular Sequence Data Models,Chemical Unilamellar Liposomes
2016/5/25
We recently described a strategy to prepare DNA-tethered lipid membranes either to fixed DNA on a surface or to DNA displayed on a supported bilayer [Boxer et al., J. Struct. Biol., 2009, 168, 190; Bo...
Stability of DNA-Tethered Lipid Membranes with Mobile Tethers
Metallic glasses Rapid-solidification Microstructure Mechanical properties
2016/5/25
We recently introduced two approaches for tethering planar lipid bilayers as membrane patches to either a supported lipid bilayer or DNA-functionalized surface using DNA hybridization (Chung, M.; Lowe...
DNA-tethered Membranes Formed by Giant Vesicle Rupture
Tethered lipid membrane Surface immobilized DNA Giant unilamellar vesicles
2016/5/25
We have developed a strategy for preparing tethered lipid bilayer membrane patches on solid surfaces by DNA hybridization. In this way, the tethered membrane patch is held at a controllable distance f...
Frictional Drag and Electrical Manipulation of Recombinant Proteins in Polymer-Supported Membranes
Polymers Lipids Recombinant Fusion Proteins Green Fluorescent Proteins Membrane Proteins Electrophoresis Membranes,Artificial Osmosis Histidine Models, Chemical
2016/5/24
We establish a lipid monolayer supported by a polymer interface that offers advantages over conventional solid-supported membranes for determining the frictional drag at the membrane-protein interface...
Phase Separation of Lipid Membranes Analyzed with High-Resolution Secondary Ion Mass Spectrometry
Cell Membrane Membrane Microdomains Membrane Lipids Lipid Bilayers Phosphatidylcholines Membrane Proteins Spectrometry, Mass, Secondary Ion Nanotechnology
2016/5/24
Lateral variations in membrane composition are postulated to play a central role in many cellular events, but it has been difficult to probe membrane composition and organization on length scales of t...
Probing the Structure of Supported Membranes and Tethered Oligonucleotides by Fluorescence Interference Contrast Microscopy
Water Oligonucleotides Microscopy, Fluorescence Microscopy, Interference Membranes, Artificial Sensitivity and Specificity Surface Properties Models, Chemical Silicon Dioxide
2016/5/24
Fluorescence interference contrast microscopy (FLIC) is a powerful method to structurally characterize fluorescent objects with nanometer-scale resolution in the z direction. Here we use FLIC to chara...
Micropattern Formation in Supported Lipid Membranes
Lipid Bilayers Phospholipids Microchemistry Diffusion Membrane Fluidity Electrochemistry
2016/5/24
Phospholipid vesicles exhibit a natural tendency to fuse and assemble into a continuous single bilayer membrane on silica and several other substrate materials. The resulting supported membrane mainta...
Arrays of Independently-Addressable Supported Fluid Bilayer Membranes
Arrays Independently Addressable Fluid Bilayer Membranes
2016/5/23
A surface detector array device suitable for use with a biosensor is disclosed. The device is formed of a substrate having a surface defining a plurality of distinct bilayer-compatible surface regions...
Cell adhesion to protein-micropatterned-supported lipid bilayer membranes
micropatterning microcontact printing of proteins supported lipid bilayers cell adhesion endothelial cells
2016/5/23
A new method for constructing controlled interfaces between cells and synthetic supported lipid bilayer membranes is reported. Microcontact printing is used to define squares and grid lines of fibrone...
Molecular Transport and Organization in Supported Lipid Membranes
Supported lipid membranes Molecular transport Membrane patterning PDMS stamp
2016/5/23
The mechanism by which vesicles spontaneously form supported lipid bilayer membranes on glass surfaces is becoming better understood and this knowledge is the basis of a technology of patterning membr...
Printing via Photolithography on Micropartitioned Fluid Lipid Membranes
Lipid membranes Liquid surfaces Micropatterning
2016/5/23
In a fluid medium there is continuous diffusive mixing among components. In order to retain spatial information such as a concentration gradient or an image in the absence of external forces, the flui...
Patterning Barriers to Lateral Diffusion in Supported Lipid Bilayer Membranes by Blotting and Stamping
Patterning Barriers Lateral Diffusion Lipid Bilayer Membranes Blotting Stamping
2016/5/23
Two new methods are introduced for patterning fluid lipid bilayer membranes on solid supports. These methods are based on the observation that supported membranes undergo self-limiting lateral expansi...
Electric Field Effects in Multicomponent Fluid Lipid Membranes
melatonin impurities bulk drug substance analytical methods impurity profile residual solvent stability purity
2016/5/23
A thermodynamic model of multicomponent fluid membranes under the influence of lateral forces is described. Membrane components are characterized by their effective molecular areas, molecular charges,...
Electric Field-Induced Critical Demixing in Lipid Bilayer Membranes
Cardiolipins Electric Stimulation Electricity Fluorescent Dyes Lipid Bilayers Membranes, Artificial Microscopy, Fluorescence Models, Chemical Models, Molecular hosphatidylcholines Phosphatidylethanolamines Thermodynamics
2016/5/23
Electric fields can induce lateral reorganization of lipids in fluid bilayer membranes. The resulting concentration profiles readily are observed in planar-supported bilayers by epifluorescence micros...