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Short Hydrogen Bonds and Proton Delocalization in Green Fluorescent Protein (GFP)
Short Hydrogen Bonds Proton Delocalization Green Fluorescent Protein GFP
2016/5/25
Short hydrogen bonds and specifically low-barrier hydrogen bonds (LBHBs) have been the focus of much attention and controversy for their possible role in enzymatic catalysis. The green fluorescent pro...
Ground-state Proton Transfer Kinetics in Green Fluorescent Protein (GFP)
Mitotic centromere-associated kinase (MCAK/Kif2C) Cellular senescence p53 Human primary cells
2016/5/25
Proton transfer plays an important role in the optical properties of green fluorescent protein (GFP). While much is known about excited-state proton transfer reactions (ESPT) in GFP occurring on ultra...
Light-Activated Reassembly of Split Green Fluorescent Protein
Tungsten particle Cobalt-cemented tungsten carbide Adhesion Gap Diamond film
2016/5/25
Truncated green fluorescent protein (GFP) with the 11th β-strand removed is potentially interesting for bioconjugation, imaging, and the preparation of semisynthetic proteins with novel spectroscopic ...
Semisynthetic green fluorescent proteins (GFPs) can be prepared by producing truncated GFPs recombinantly and assembling them with synthetic beta-strands of GFP. The yield from expressing the truncate...
Deconstructing Green Fluorescent Protein
Green Fluorescent Proteins Spectrometry, Fluorescence Protein Structure,Secondary Protein Folding Peptide Fragments Models,Molecular
2016/5/25
Green fluorescent protein (GFP) has been reassembled from two pieces, a large fragment 214 amino acids in length that is produced recombinantly (GFP 1-10) and a short synthetic peptide corresponding t...
Dynamic Stokes Shift in Green Fluorescent Protein Variants
GFP solvation response ultrafast
2016/5/25
Solvent reorganization around the excited state of a chromophore leads to an emission shift to longer wavelengths during the excited-state lifetime. This solvation response is absent in wild-type gree...
Anomalous Negative Fluorescence Anisotropy in Yellow Fluorescent Protein (YFP 10C):Quantitative Analysis of FRET in YFP Dimers
Alzheimer's disease Amyloid beta 40 peptide Human serum albumin Circular dichroism Dissociation constant alpha-Helix
2016/5/25
Yellow fluorescent protein (YFP) is widely used as a genetically encoded fluorescent marker in biology. In the course of a comprehensive study of this protein, we observed an unusual, negative fluores...
Ultrafast Excited-State Dynamics in the Green Fluorescent Protein Variant S65T/H148D.2.Unusual Photophysical Properties
Arabidopsis thaliana homogentisate phytyltransferase (HPT/VTE2) Lactuca sativa tocopherol tocopherol cyclase (TC/VTE1) vitamin e
2016/5/25
In the preceding accompanying paper [Shu, X., et al. (2007) Biochemistry 46, 12005-12013], the 1.5 A resolution crystal structure of green fluorescent protein (GFP) variant S65T/H148D is presented, an...
Ultrafast Excited-State Dynamics in the Green Fluorescent Protein Variant S65T/H148D.1.Mutagenesis and Structural Studies
Arabidopsis thaliana homogentisate phytyltransferase (HPT/VTE2) Lactuca sativa tocopherol tocopherol cyclase (TC/VTE1) vitamin e.
2016/5/24
Wild type green fluorescent protein (wt-GFP) and the variant S65T/H148D each exhibit two absorption bands, A and B, which are associated with the protonated and deprotonated chromophores, respectively...
Enhancement of the Fluorescence of the Blue Fluorescent Proteins by High Pressure or Low Temperature
Enhancement Fluorescence Blue Fluorescent Proteins High Pressure Low Temperature
2016/5/24
Green fluorescent proteins bearing the Y66H mutation exhibit strongly blue-shifted fluorescence excitation and emission spectra. However, these blue fluorescent proteins (BFPs) have lower quantum yiel...
Green Fluorescent Protein Variants as Ratiometric Dual Emission pH Sensors.3.Temperature Dependence of Proton Transfer
Green Fluorescent Proteins Amino Acid Substitution Spectrometry, Fluorescence Kinetics Mutagenesis,Site-Directed Models,Theoretical Genetic Variation Hydrogen-Ion Concentration Thermodynamics Solvents
2016/5/24
In parts 1 and 2 of this series [Hanson, G. T., McAnaney, T. B., Park, E. S., Rendell, M. E. P., Yarbrough, D. K., Chu, S. Y., Xi, L. X., Boxer, S. G., Montrose, M. H., and Remington, S. J. (2002) Bio...
Protonation,Photobleaching,and Photoactivation of Yellow Fluorescent Protein (YFP 10C):A Unifying Mechanism
Protonation Photobleaching Photoactivation Yellow Fluorescent Protein Unifying Mechanism
2016/5/24
Yellow fluorescent protein (YFP 10C) is widely used as a probe in biology, but its complex photochemistry gives rise to unusual behavior that requires fuller definition. Here we characterize the kinet...
Polarized Absorption Spectra of Green Fluorescent Protein Single Crystals:Transition Dipole Moment Directions
Animals Scyphozoa Protons Threonine Serine Luminescent Proteins Green Fluorescent Proteins Microscopy, Fluorescence Amino Acid Substitution Spectrometry, Fluorescence
2016/5/24
Polarized absorption spectra of orthorhombic crystals of wild-type green fluorescent protein (GFP) were measured between 350 and 520 nm to obtain information on the directions of the electronic transi...
Green Fluorescent Protein Variants as Ratiometric Dual Emission pH Sensors.2.Excited-State Dynamics
Animals Protons Deuterium Luminescent Proteins Green Fluorescent Proteins Spectrometry, Fluorescence Biosensing Techniques Kinetics Genetic Variation Hydrogen-Ion Concentration
2016/5/24
In the preceding paper [Hanson, G. T., McAnaney, T. B., Park, E. S., Rendell, M. E. P., Yarbrough, D. K., Chu, S., Xi, L., Boxer, S. G., Montrose, M. H., and Remington, S. J. (2002) Biochemistry 41, 1...
Green Fluorescent Protein Variants as Ratiometric Dual Emission pH Sensors.1.Structural Characterization and Preliminary Application
Animals Cricetulus Cell Line Threonine Luminescent Proteins Green Fluorescent Proteins Microscopy, Confocal Crystallography, X-Ray Transfection Amino Acid Substitution
2016/5/24
Novel dual emission, pH-sensitive variants of the green fluorescent protein (GFP) have been constructed and are suitable for ratiometric emission measurements in vivo. This new class of GFPs, termed d...